Method for measuring substance and testing piece

• US 7,098,038 B2
• Filed: 03/11/2003
• Issued: 08/29/2006
• Est. Priority Date: 10/30/1995
• Status: Expired due to Fees

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Fukuoka, Takao, Katayama, Atsuko
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US10/384,577
• Publication Number:
  US 20030175984A1
• Time in Patent Office: 1,267 Days
• Field of Search: 422/56, 422/57, 436/164, 436/169, 436/178
• US Class Current: 436/164
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 31/00 Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/23 Carbon containing
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

Claims

1. A method for measuring an analyte in a sample, comprising:

a step of forming a detectable substance based on a chemical reaction of the analyte in the sample, in a liquid phase reaction system which comprises a reaction solvent and a layered inorganic compound dispersed in the reaction system, wherein at least one substance insoluble in the reaction solvent is formed in the chemical reaction, wherein the substance insoluble in the reaction solvent is absorbed to the layered inorganic compound, wherein precipitation of the substance insoluble in the reaction solvent is suppressed, and wherein the substance insoluble in the reaction solvent is dispersed in the reaction system; and

a step of measuring the detectable substance, thereby measuring the analyte.

2. The method according to claim 1, wherein the layered inorganic compound is a 2:

1 type clay mineral.

3. The method according to claim 2, wherein the 2:

1 type clay mineral is a swelling layered clay mineral.

4. The method according to claim 3, wherein the swelling layered clay mineral is at least one member selected from the group consisting of bentonite, smectite, vermicullite and synthetic fluoro-mica.

5. The method according to claim 4, wherein the smectite is synthetic smectite.

6. The method according to claim 5, wherein the synthetic smectite is at least one member selected from the group consisting of hectorite and saponite.

7. The method according to claim 1, wherein the layered inorganic compound is dispersed in a reaction medium of the reaction system in the form of any one selected from the group consisting of dispersion, sol, gel, slurry, agglomerate, aggregate and sintered porous body.

8. The method according to claim 7, wherein the reaction medium in which the layered inorganic compound is dispersed is the reaction solvent of the formation reaction of the substance insoluble in the reaction solvent.

9. The method according to claim 1, wherein the insoluble substance is the detectable substance which is a dyestuff detectable by an optical method.

10. The method according to claim 1, wherein the detectable substance is a compound selected from the group consisting of amines, imines, polyenes, aromatic compounds, heterocyclic compounds and complexes between ions and cyclic ligands.

11. The method according to claim 1, wherein the formation reaction of the detectable substance is an oxidation-reduction reaction.

12. The method according to claim 11, wherein the formation reaction of the detectable substance is a reduction reaction of a tetrazolium salt.

13. The method according to claim 1, wherein a buffer or a dried product thereof is contained in the reaction system.

14. The method according to claim 1, wherein the layered inorganic compound is dispersed uniformly in the reaction solvent to adsorb the insoluble substance to the layered inorganic compound.