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In situ maturation of cultured pancreatic stem cells having a specified, intermediate stage of development

  • US 7,101,546 B2
  • Filed: 12/20/2002
  • Issued: 09/05/2006
  • Est. Priority Date: 12/21/2001
  • Status: Active Grant
First Claim
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1. A method of providing insulin to a mammal comprising the steps of:

  • (a) encapsulating a cell culture of propagating pancreatic cells having the following properties;

    (i) the cells have the ability to be passed from one culture vessel to a second vessel at an initial concentration of about 180 cells per square centimeter and expanded to about 1,800 cells per square centimeter;

    (ii) both the unexpanded and expanded cells are 90% PDX-1 positive and have an insulin;

    actin mRNA ratio of between 1;

    100 and 1000;

    1; and

    (iii) the cells mature into an aggregate of cells, comprising an encapsulating mantle of CK19-positive cells and an inner cell mass, wherein the aggregate comprises 50–

    5000 pancreatic cells and has a diameter of between 50 and 300 microns; and

    ,(b) inserting the encapsulated cells into the mammal, whereby the encapsulated cells further mature to insulin secreting cells and provide insulin to the mammal.

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