Method and probes for the detection of chromosome aberrations
First Claim
1. A method of determining the presence or absence of chromosomal aberrations in a sample of eukaryotic origin comprising the steps of:
- a) contacting said sample with a hybridization solution comprising at least two sets of hybridization probes, at least one first set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking one side of a potential breakpoint in a chromosome, and at least one second set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking the other side of the potential breakpoint, wherein the first set of probes is labeled with at least one first specific label while the second set of probes is labeled with at least one second specific label different from the first specific label, and wherein the two sets of hybridization probes hybridize such that the first and second specific labels produce a fused signal if no chromosome break is present;
b) removing any unbound and any non-specifically bound probe; and
c) detecting the hybridized probes, and thereby determining the presence or absence of chromosomal aberrations in the sample.
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Abstract
A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.
207 Citations
19 Claims
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1. A method of determining the presence or absence of chromosomal aberrations in a sample of eukaryotic origin comprising the steps of:
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a) contacting said sample with a hybridization solution comprising at least two sets of hybridization probes, at least one first set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking one side of a potential breakpoint in a chromosome, and at least one second set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking the other side of the potential breakpoint, wherein the first set of probes is labeled with at least one first specific label while the second set of probes is labeled with at least one second specific label different from the first specific label, and wherein the two sets of hybridization probes hybridize such that the first and second specific labels produce a fused signal if no chromosome break is present; b) removing any unbound and any non-specifically bound probe; and c) detecting the hybridized probes, and thereby determining the presence or absence of chromosomal aberrations in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification