Sequencing by incorporation
First Claim
1. A method for sequencing a nucleic acid, the method comprising:
- (i) providing a nucleic acid template and a primer;
(ii) incubating the nucleic acid template and the primer with a polymerase and one or more nucleotide analogs having formula (III),
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Accused Products
Abstract
Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
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Citations
21 Claims
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1. A method for sequencing a nucleic acid, the method comprising:
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(i) providing a nucleic acid template and a primer; (ii) incubating the nucleic acid template and the primer with a polymerase and one or more nucleotide analogs having formula (III), - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification