Pressure-based mobility shift assays
First Claim
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1. A method of performing a mobility shift assay in a microfluidic device, the method comprising:
- flowing a reaction mixture comprising an enzyme, an enzyme substrate, and a product through a separation region of the microfluidic device under an applied pressure, which separation region comprises at least one ion-exchange material comprising a polyacrylamide material or a dimethylacrylamide material modified by one or more additives having formula (I), or;
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Abstract
Methods for chromatographically separating materials, including the separation of materials in kinase or phosphatase assays, in microfluidic devices under positive or negative fluid pressure. Devices and integrated systems for performing chromatographic separations are also provided.
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Citations
31 Claims
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1. A method of performing a mobility shift assay in a microfluidic device, the method comprising:
flowing a reaction mixture comprising an enzyme, an enzyme substrate, and a product through a separation region of the microfluidic device under an applied pressure, which separation region comprises at least one ion-exchange material comprising a polyacrylamide material or a dimethylacrylamide material modified by one or more additives having formula (I), or; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
Specification