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Pressure-based mobility shift assays

  • US 7,105,304 B1
  • Filed: 11/05/2001
  • Issued: 09/12/2006
  • Est. Priority Date: 11/07/2000
  • Status: Expired due to Term
First Claim
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1. A method of performing a mobility shift assay in a microfluidic device, the method comprising:

  • flowing a reaction mixture comprising an enzyme, an enzyme substrate, and a product through a separation region of the microfluidic device under an applied pressure, which separation region comprises at least one ion-exchange material comprising a polyacrylamide material or a dimethylacrylamide material modified by one or more additives having formula (I), or;

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