Microfluidic chip with enhanced tip for stable electrospray ionization

• US 7,105,812 B2
• Filed: 06/18/2004
• Issued: 09/12/2006
• Est. Priority Date: 08/26/2003
• Status: Expired due to Fees

• Current Assignee: Vern Norviel
• Original Assignee: Predicant Biosciences, Inc. (Response Genetics, Inc)
• Inventors: Ni, Jing, Zhao, Mingqi, Bousse, Luc, Blaga, Iuliu, Stults, John
• Primary Examiner(s): Wells, Nikita
• Application Number:
  US10/871,498
• Publication Number:
  US 20050047969A1
• Time in Patent Office: 816 Days
• Field of Search: 250/288, 250/282, 250/281, 250/285, 250/428, 422/100
• US Class Current: 250/288
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/027 electrostatic forces between substrate and tip
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B81B 1/008 Microtips
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: H01J 49/0018 Microminiaturised spectrometers, e.g. chip-integrated devices, Micro-Electro-Mechanical Systems [MEMS]
• CPC Class Codes: H01J 49/167 Capillaries and nozzles specially adapted therefor; electrostatic spraying per se B05B5/00

Abstract

A microfluidic chip formed with multiple fluid channels terminating at a tapered electrospray ionization tip for mass spectrometric analysis. The fluid channels may be formed onto a channel plate that are in fluid communication with corresponding reservoirs. The electrospray tip can be formed along a defined distal portion of the channel plate that can include a single or multiple tapered surfaces. The fluid channels may terminate at an open-tip region of the electrospray tip. A covering plate may substantially enclose most portions of the fluid channels formed on the channel plate except for the open-tip region. Another aspect of the invention provides methods for conducting mass spectrometric analysis of multiple samples flowing through individual fluid channels in a single microfluidic chip that is formed with a tapered electrospray tip having an open-tip region.

Claims

1. A microfluidic chip for mass spectrometric analysis comprising:

a microfluidic body layer formed with at least one fluid reservoir;

at least one defined microchannel formed along the microfluidic body layer that is in fluid communication with the fluid reservoir; and

a laminate layer for enclosing the defined microchannel formed along the microfluidic body layer, wherein the laminate layer extends beyond a tapered end portion of the microfluidic body layer to form a open-ended distal tip portion at which the defined microchannel terminates to provide an electrospray ionization tip that directs an ionization spray from the microfluidic chip.

2. The microfluidic chip as recited in claim 1, wherein at least a portion of the open-ended distal tip portion is covered with a hydrophilic material.

3. The microfluidic chip as recited in claim 1, wherein the tapered end portion of the microfluidic body layer is a two-dimensional taper formed along a relative X-Y axis and a relative Y-Z axis.

4. The microfluidic chip as recited in claim 1, wherein the tapered end portion of the microfluidic body layer includes a tapered end formed along a substantially flat truncated portion of the tapered end portion.

5. The microfluidic chip as recited in claim 4, wherein the tapered end has a thickness of less than 500 μ

m.

6. The microfluidic chip as recited in claim 1, wherein the microfluidic body layer includes an extended platform section having a flat tapered portion.

7. The microfluidic chip as recited in claim 6, wherein the flat tapered portion of the extended platform section has a thickness of less than 500 μ

m.

8. The microfluidic chip as recited in claim 1, wherein a plurality of microchannels are formed along the microfluidic body layer that are enclosed by the laminate layer to provide an electrospray ionization tip whereby each microchannel terminates to direct an ionization spray from the microfluidic chip.

9. The microfluidic chip as recited in claim 1, wherein the laminate layer is formed with a distal tip groove.

10. A microfluidic chip for electrospray ionization comprising:

a channel plate formed with at least two non-intersecting fluid channels that are each in fluid communication with at least one fluid reservoir also included with the channel plate; and

a covering plate for substantially enclosing the non-intersecting fluid channels formed on the channel plate, wherein the covering plate includes an overhang that extends beyond a tapered end portion of the channel plate to provide an electrospray tip that includes an open-tip region at which each of the non-intersecting fluid channels terminate.

11. The microfluidic chip as recited in claim 10, wherein the tapered end portion of the channel plate is dual-tapered and formed along two-dimensions defined by a relative X-Y axis and a relative Y-Z axis.

12. The microfluidic chip as recited in claim 10, wherein the electrospray tip is formed within a recessed portion of the microfluidic chip.

13. The microfluidic chip as recited in claim 12, wherein the electrospray tip is formed with a sharp point that is protected within the recessed portion of the microfluidic chip.

14. The microfluidic chip as recited in claim 10, wherein the covering plate is formed with a tip groove.

15. The microfluidic chip as recited in claim 10, wherein the channel plate and covering plate are formed of a material selected from at least one of the following:

a polymer, a copolymer, an elastomer, a ceramic, quartz, silicon, silicon dioxide, silica, and glass.

16. The microfluidic chip as recited in claim 10, wherein the channel plate is defined with a first side and second opposite side separated along a longitudinal axis, and wherein at least one fluid channel is formed on the first side of the channel plate and at least one fluid channel is formed on the second opposite side of the channel plate.

17. A microfluidic chip for mass spectrometric analysis comprising:

a body layer formed with a plurality of reservoirs for containing at least one fluid medium, wherein a plurality of separated channels are formed within the body layer that are each in fluid communication with at least one reservoir but not in fluid communication with each other within at least a portion of the body layer; and

an enclosure layer for enclosing at least a portion of the separated channels formed within the body layer, wherein the enclosure layer further includes an exposed open-tip extension extending beyond a dual-tapered end portion of the body layer to provide an electrospray ionization tip at which the plurality of separated channels terminate to direct an ionization spray from the microfluidic chip.

18. The microfluidic chip as recited in claim 17, wherein the exposed open-tip extension and the enclosure layer are formed as a single-piece to provide a monolithic electrospray tip.

19. The microfluidic chip as recited in claim 17, wherein the exposed open-tip extension and the enclosure layer are separate pieces that are joined together.

20. The microfluidic chip as recited in claim 17, wherein the exposed open-tip extension is formed with a groove tip.

21. A microfluidic chip with enhanced electrospray tip for mass spectrometric analysis comprising:

a microfluidic chip formed with at least one fluid reservoir for containing at least one fluid medium;

at least one microchannel channel formed within the microfluidic chip that is in fluid communication with the fluid reservoir; and

an electrospray ionization tip formed with a tapered open-ended distal tip portion positioned along an end surface of the microfluidic chip, wherein the microchannel terminates at the open-ended distal tip portion to direct an ionization spray from the microfluidic chip.