Reversible binding of molecules to metal substrates through affinity interactions

• US 7,108,971 B2
• Filed: 12/19/2001
• Issued: 09/19/2006
• Est. Priority Date: 05/14/1999
• Status: Expired due to Fees

• Current Assignee: Iris Technologies, Inc.
• Original Assignee: Iris Technologies, Inc.
• Inventors: Ramirez-Vick, Jaime E.
• Primary Examiner(s): Kim, Young J.
• Application Number:
  US10/029,113
• Publication Number:
  US 20020160391A1
• Time in Patent Office: 1,735 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/16, 536/300, 536/23.1, 536/24.3, 536/24.33
• US Class Current: 435/6.11
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

This invention is related to methods for the immobilization of labeled ligands on solid surfaces using soft metal-soft base bonding. The ligand-binding solid surface comprises a soft metal solid support and a heterobifunctional spacer chemi- or physisorbed to said soft metal solid support via soft metal-soft base bonding.

Claims

1. A ligand-binding solid surface comprising:

a) a soft metal solid support and

b) a heterobifunctional spacer having two functional groups, one of said functional groups comprising a reactive terminal moiety configured to bind a ligand, and one of said functional groups comprising a soft base, said spacer being non-covalently chemi- or physisorbed to said soft metal solid support via soft metal-soft base bonding wherein the soft base is selected from the group consisting of succinimidyl-6-(biotinamido)hexanoate and succinimidyl 6-[6-(((iodoacetyl)amino)-hexanoyl)amino]hexanoate.

2. A ligand-binding solid surface of claim 1 in which the soft metal solid support is selected from the group consisting of silver, copper, gold, platinum (II), mercury, mercury (II), thallium, cadmium (II), platinum (IV) and palladium (II) covered surfaces.

3. A ligand-binding solid surface of claim 1 in which the heterobifunctional spacer comprises a hydrocarbon having a chain length of about 10 to about 40 carbon atoms.

4. A ligand-binding solid surface of claim 1 wherein the heterobifunctional spacer comprises succinimidyl-6-(biotinamido)hexanoate.

5. An assay system comprising a plurality of ligand-binding solid surfaces of claim 1.

6. A method for detecting the presence of a biological molecule comprising exposing a sample containing biological molecules to a ligand-binding solid surface of claim 1.

7. A ligand-binding solid surface of claim 1 wherein said reactive terminal moiety comprises an oligonucleotide linked to said heterobifunctional spacer.

8. A ligand-binding solid surface of claim 1 in which the heterobifunctional spacer comprises succinimidyl 6-[6-(((iodoacetyl)amino)-hexanoyl)amino]hexanoate.

9. A method for preparing a ligand-binding solid surface, comprising:

a) selecting a soft metal solid support; and

b) non-covalently immobilizing a heterobifunctional spacer on said solid support via soft metal-soft base bonding, said spacer having two functional groups, one of said functional groups comprising a reactive terminal moiety configured to bind a ligand, and one of said functional groups comprising a soft base, wherein the soft base is selected from the group consisting of succinimidyl-6-(biotinamido)hexanoate and succinimidyl 6-[6-(((iodoacetyl)amino)-hexanoyl)amino]hexanoate.

10. A method of claim 9 in which the soft metal solid support is selected from the group consisting of silver, copper, gold, platinum (II), mercury, mercury (II), thallium, cadmium (II), platinum (IV) and palladium (II) covered surfaces.

11. A method of claim 9 in which the heterobifunctional spacer comprises a hydrocarbon of about 10 to about 40 atoms in length.

12. A method of claim 9 wherein the heterobifunctional spacer comprises succinimidyl-6-(biotinamido)hexanoate.

13. A method of claim 9 wherein the heterobifunctional spacer comprises succinimidyl 6-[6-(((iodoacetyl)amino)-hexanoyl)amino]hexanoate.