Biomolecule-bound substrates

• US 7,109,024 B2
• Filed: 04/07/2003
• Issued: 09/19/2006
• Est. Priority Date: 11/15/1999
• Status: Expired due to Fees

• Current Assignee: DR. Chip Biotechnology, Inc.
• Original Assignee: DR. Chip Biotechnology, Inc.
• Inventors: Hsiao, Hsiung, Shih, Yu-Hau, Lee, Kan-Hung, Wang, Yih-Weng, Tsai, Chuan-Mei, Bair, Chi-Horng, Wang, Shin-Hwan
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US10/408,519
• Publication Number:
  US 20030228683A1
• Time in Patent Office: 1,261 Days
• Field of Search: 436/518, 436/524, 436/527, 436/529, 436/530, 436/531, 435/40.5, 435/40.51, 435/283.1, 435/288.3, 435/6, 435/287.2, 422/21, 422/68.1, 427/508, 427/553, 427/586, 427/595, 522 1- 5, 523/137, 530/333, 530/334, 428/480, 428/492, 428/497, 428/532
• US Class Current: 435/287.2
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof

Abstract

This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.

Claims

1. A nucleic acid-bound substrate comprising:

a support made of an organic polymer and having an unmodified surface; and

a plurality of unmodified nucleic acids immobilized on the unmodified surface;

wherein the organic polymer is acrylic resin, polyethylene, polysulfone, cellulose acetate, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof.

2. The substrate of claim 1, wherein the organic polymer is acrylic resin, polyethylene, cellulose acetate, or a combination thereof.

3. The substrate of claim 2, wherein the unmodified surface is non-porous.

4. The substrate of claim 3, wherein the organic polymer is acrylic resin.

5. A nucleic acid-bound substrate comprising:

a support made of an organic polymer and having an unmodified non-porous surface; and

a plurality of unmodified nucleic acids immobilized on the unmodified non-porous surface;

wherein the organic polymer is acrylic resin, polyethylene, polysulfone, cellulose acetate, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof.

6. A method of preparing a biomolecule-bound substrate, comprising:

placing a plurality of unmodified biomolecules on an unmodified surface of a support made of an organic polymer; and

irradiating the unmodified biomolecules and the unmodified surface with ultraviolet light;

wherein the organic polymer is acrylic resin, polyethylene, polysulfone, cellulose acetate, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof.

7. The method of claim 6, wherein the organic polymer is acrylic resin, polyethylene, cellulose acetate, or a combination thereof.

8. The method of claim 7, wherein the unmodified surface is non-porous.

9. The method of claim 8, wherein the organic polymer is acrylic resin.

10. The method of claim 6, wherein the unmodified biomolecules are polypeptides.

11. The method of claim 10, wherein the organic polymer is acrylic resin, polyethylene, cellulose acetate, or a combination thereof.

12. The method of claim 11, wherein the unmodified surface is non-porous.

13. The method of claim 12, wherein the organic polymer is acrylic resin.

14. The method of claim 6, wherein the unmodified biomolecules are nucleic acids.

15. The method of claim 14, wherein the organic polymer is acrylic resin, polyethylene, cellulose acetate, or a combination thereof.

16. The method of claim 15, wherein the unmodified surface is non-porous.

17. The method of claim 16, wherein the organic polymer is acrylic resin.

18. A method of preparing a biomolecule-bound substrate, comprising:

placing a plurality of unmodified biomolecules on an unmodified non-porous surface of a support made of organic polymer; and

irradiating the unmodified biomolecules and the unmodified non-porous surface with ultraviolet light;

wherein the organic polymer is acrylic resin, polyethylene, polysulfone, cellulose acetate, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof.

19. The method of claim 18, wherein the unmodified biomolecules are polypeptides.

20. The substrate of claim 18, wherein the unmodified biomolecules are nucleic acids.