Method and apparatus for analyzing multi-channel chromatogram

• US 7,110,886 B2
• Filed: 07/18/2005
• Issued: 09/19/2006
• Est. Priority Date: 03/28/1994
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Ito, Masahito, Deguchi, Kisaburo
• Primary Examiner(s): Tsai, Carol S. W.
• Application Number:
  US11/182,855
• Publication Number:
  US 20050251349A1
• Time in Patent Office: 428 Days
• Field of Search: None
• US Class Current: 702/22
• CPC Class Codes: G01N 2201/1293 resolving multicomponent spectra
• CPC Class Codes: G01N 30/8624 Detection of slopes or peaks; baseline correction
• CPC Class Codes: G01N 30/8631 Peaks
• CPC Class Codes: G01N 30/8679 Target compound analysis, i.e. whereby a limited number of peaks is analysed

Abstract

A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Claims

1. A method of analyzing overlapping peaks on a multi-channel chromatogram, comprising the steps of:

deconvoluting said multi-channel chromatogram using a predetermined dispersion function;

multivariate-analyzing the deconvoluted multi-channel chromatogram;

determining whether multivariate analysis corresponding to the width of said predetermined dispersion function uniquely has a solution;

adjusting the width of said dispersion function by a subtraction operation subtracting a standard deviation from said width of said dispersion function until the multivariate analysis uniquely has a solution; and

determining a multi-channel chromaogram deconvoluted by using a dispersion function adjusted by said subtraction operation.

2. A method of analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the step of measuring peak sizes in the determined multi-channel chromatogram and performing a quantitative analysis.

3. A method of analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the step of measuring retention times on the basis of the determined multi-channel chromatogram and performing a qualitative analysis.

4. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the step of reconvoluting the deconvoluted multi-channel chromatogram, measuring peak sizes of the reconvoluted multi-channel chromatogram and performing a quantitative analysis.

5. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, measuring retention times of the reconvoluted multi-channel chromatogram and performing a qualitative analysis.

6. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the steps of compressing the deconvoluted multi-channel chromatogram, measuring peak sizes of the compressed multi-channel chromatogram and performing a quantitative analysis.

7. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the steps of compressing the deconvoluted multi-channel chromatogram, measuring retention times of the compressed multi-channel chromatogram and performing a qualitative analysis.

8. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, compressing the reconvoluted multi-channel chromatogram, measuring peak sizes of the compressed multi-channel chromatogram and performing a quantitative analysis.

9. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 1, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, compressing the reconvoluted multi-channel chromatogram, measuring retention times of the compressed multi-channel chromatogram and performing a qualitative analysis.

10. A method of analyzing overlapping peaks on a multi-channel chromatogram, comprising the steps of:

deconvoluting said multi-channel chromatogram using a predetermined dispersion function;

multivariate-analyzing the deconvoluted multi-channel chromatogram;

determining whether multivariate analysis corresponding to the width of said predetermiend dispersion function uniquely has a solution;

adjusting said dispersion function by a deconvolution operation with changing a deconvoluting parameter from said dispersion function until the multivariate analysis uniquely has a solution; and

determining a multi-channel chromatogram deconvoluted by using a dispersion function adjusted by said deconvolution operation.

11. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the step of measuring peak sizes in the determined multi-channel chromatogram and performing a quantitative analysis.

12. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the step of measuring retention times on the basis of the determined multi-channel chromatogram and performing a qualitative analysis.

13. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, and measuring peak sizes of the reconvoluted multi-channel chromatogram and performing a quantitative analysis.

14. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, and measuring retention times of the reconvoluted multi-channel chromatogram and performing a qualitative analysis.

15. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of compressing the deconvoluted multi-channel chromatogram, and measuring peak sizes of the compressed multi-channel chromatogram and performing a quantitative analysis.

16. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of compressing the deconvoluted multi-channel chromatogram, and measuring retention times of the compressed multi-channel chromatogram and performing a qualitative analysis.

17. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, compressing the reconvoluted multi-channel chromatogram, and measuring peak sizes of the compressed multi-channel chromatogram and performing a quantitative analysis.

18. A method for analyzing overlapping peaks on a multi-channel chromatogram according to claim 10, further comprising the steps of reconvoluting the deconvoluted multi-channel chromatogram, compressing the reconvoluted multi-channel chromatogram, and measuring retention times of the compressed multi-channel chromatogram and performing a qualitative analysis.