Biosensor

• US 7,112,451 B2
• Filed: 06/28/2001
• Issued: 09/26/2006
• Est. Priority Date: 06/28/2000
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Tanaka, Hirotaka, Kitawaki, Fumihisa, Takahashi, Mie, Nadaoka, Masataka
• Primary Examiner(s): NGUYEN, BAO THUY L
• Application Number:
  US10/069,845
• Publication Number:
  US 20020164822A1
• Time in Patent Office: 1,916 Days
• Field of Search: 436/514, 436/518, 436/810, 435/4, 435/7.1, 435/7.94, 435/287.1, 435/287.2, 435/287.3, 435/287.7, 435/287.9, 435/805, 435/810, 435/970, 422 56- 60
• US Class Current: 436/514
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A biosensor is provided with an area in which a reagent having bleaching action is carried in a state where it can be dissolved, at least in a part of a sample application area to which an inspection target solution is applied or the downstream of the sample application area in the direction of the inspection target solution permeating, in a development layer. In the so-constituted biosensor, a colored component in the inspection target solution can be faded by the bleaching reagent, so that the color in parts other than a reactive area is reduced in consequence, whereby a visual judgment is possible and a more accurate measurement result in which a reading error by a measuring device is extremely suppressed can be obtained.

Claims

1. A biosensor comprising a development layer where an inspection target solution is developed, and holding at least a marker reagent part where a marker reagent is held so as to be dissolved by the development of the inspection target solution in a part of the development layer, and a reagent immobilization part where a reagent which specifically reacts to an analysis target in the inspection target solution is immobilized in a part that is chromatographically downstream relative to the marker reagent part of the development layer;

the development layer is provided with a bleaching reagent area where a bleaching reagent having bleaching action is supported in a dry state where it can be dissolved, at least in a part of a sample application area where the inspection target solution is applied or downstream of the sample application area in the permeating direction of the inspection target solution;

wherein, the bleaching reagent is sodium percarbonate.

2. The biosensor as defined in claim 1, wherein the development layer is made of nitrocellulose.

3. The biosensor as defined in claim 1, wherein the bleaching reagent is directly carried on the development layer so as to be dissolved.

4. The biosensor as defined in claim 1, wherein a sample inflow area to which the inspection target solution flows in by a capillary phenomenon is arranged on the development layer, and the bleaching reagent area is held in the sample inflow area.

5. The biosensor as defined in claim 1, wherein the inspection target solution to be applied is whole blood.

6. The biosensor as defined in claim 1, wherein the biosensor is a one-step immunochromatographic test strip.

7. The biosensor as defined in claim 1, wherein the biosesor is a dry analysis element.