Bio-imaging and information system for scanning, detecting, diagnosing and optimizing plant health

• US 7,112,806 B2
• Filed: 09/26/2002
• Issued: 09/26/2006
• Est. Priority Date: 09/27/2001
• Status: Expired due to Fees

• Current Assignee: Robert Lussier
• Original Assignee: Robert Lussier
• Inventors: Lussier, Robert
• Primary Examiner(s): Gabor, Otilia
• Assistant Examiner(s): Sung, Christine
• Application Number:
  US10/255,225
• Publication Number:
  US 20050072935A1
• Time in Patent Office: 1,461 Days
• Field of Search: 250/458.1, 250/459.1, 250/461.2, 250/461.1, 356/317, 356/318, 356/417
• US Class Current: 250/458.1
• CPC Class Codes: G01N 2021/635 Photosynthetic material analysis, e.g. chrorophyll
• CPC Class Codes: G01N 2021/8466 Investigation of vegetal material, e.g. leaves, plants, fruits
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health. The system includes an enclosure for placement around a plant to be imaged in-situ. There is a controlled light source that controllably provides to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure. The chlorophyll fluorescence emitted from the plant both spatially and temporally is captured, and the captured fluorescence information is analyzed to provide plant health information.

Claims

1. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health, comprising:

an enclosure for placement around a plant to be imaged in-situ;

means for controlling a light source and providing to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure;

means for capturing chlorophyll fluorescence emitted from the plant both spatially and temporally, the means for capturing comprising an imaging sensor with a plurality of pixels defining an area sensor, to create an area fluorescence emission image of the plant, and means for storing in memory the output of the area sensor pixels over time, to form a data cube comprising the area fluorescence emission images saved over time;

means for allowing a user to select a pixel from those pixels for which data has been stored, and in response displaying for the user a graphic plot of the fluorescence intensity for the selected pixel over time and the first integral of said intensity-time; and

means for analyzing the plotted fluorescence information to determine at least the chlorophyll yield and the plant'"'"'s stress, wherein the shape of the fluorescence intensity curve and the value of the first integral indicate whether the plant is in excellent health, with a relatively flat curve and a relatively low first integral value, or the plant is in stress, with a curve having a peak and a greater first integral value.

2. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 1 in which the area sensor captures energy in the red and new-infrared spectral hands.

3. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 1 further comprising means for allowing a user to select a location in a saved image, and means for reporting to the user the measured fluorescence at the selected location.

4. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 1 in which the light source comprises a lamp, and the means for controlling the light source comprises a shutter for controllably blocking light from the lamp from reaching the plant, and providing the lamp light to the plant.

5. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 4 in which the means for controlling the light source further comprises means for controlling the shutter position.

6. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 1 in which the light source comprises a plurality of light emitting diodes, and the means for controlling the light source comprises means for controlling the power to the light emitting diodes.

13. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 1 in which the light source comprises a lamp, and the means for controlling the light source comprises a shutter for controllably blocking light from the lamp from reaching the plant, and providing the lamp light to the plant.

14. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 13 in which the means for controlling the light source further comprises means for controlling the shutter position.

7. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health, comprising:

an enclosure for placement around a plant to be imaged in-situ;

means for controlling a light source and providing to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure;

an imaging sensor that captures energy in the red and near-infrared spectral bands with a plurality of pixels defining an area sensor to create an area fluorescence emission image of the plant, and means for storing in memory the output of the area sensor pixels over time, to form a data cube comprising the area fluorescence emission images saved over time, for capturing chlorophyll fluorescence emitted from the plant both spatially and temporally;

means for allowing a user to select a pixel from those pixels for which data has been stored, and in response displaying for the user a graphic plot of the fluorescence intensity for the selected pixel over time and the first integral of said intensity-time; and

means for analyzing the plotted fluorescence information to determine at least the chlorophyll yield and the plant'"'"'s stress, wherein the shape of the fluorescence intensity curve and the value of the first integral indicate whether the plant is in excellent health, with a relatively flat curve and a relatively low first integral value, or the plant is in stress, with a curve having a peak and a greater first integral value.

8. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system for use in determining plant health, comprising:

an enclosure for placement around a plant to be imaged in-situ;

means for controlling a light source and providing to the plant light of a desired wavelength range, to controllably irradiate the plant within the enclosure;

means for capturing chlorophyll fluorescence emitted from the plant both spatially and temporally;

means for allowing a user to select a pixel from those pixels for which data has been stored, and in response displaying for the user a graphic plot the fluorescence intensity for the selected pixel over time and the first integral of said intensity-time; and

means for analyzing the plotted fluorescence information, to determine at least the chlorophyll yield and the plant'"'"'s stress, wherein the shape of the fluorescence intensity curve and the value of the first integral indicate whether the plant is in excellent health, with a relatively flat curve and a relatively low first integral value, or the plant is in stress, with a curve having a peak and a greater first integral value.

9. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 8 in which the means for capturing includes an imaging sensor with a plurality of pixels defining an area sensor, to create an area fluorescence image of the plant.

10. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 9 in which the means for capturing further includes means for storing in memory the output of the sensor pixels over time, to form a data cube comprising the area fluorescence emission images saved over time.

11. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 8 in which the area sensor captures energy in the red and near-infrared spectral bands.

12. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 8 further comprising means for allowing a user to select a location in a saved image, and means for reporting to the user the measured fluorescence at the selected location.

15. A portable Chlorophyll Fluorescence Imaging Time (CFIT) system of claim 8 in which the light source comprises a plurality of light emitting diodes, and the means for controlling the light source comprises means for controlling the power to the light emitting diodes.