Multimode reader

• US 7,113,285 B2
• Filed: 12/09/2003
• Issued: 09/26/2006
• Est. Priority Date: 12/09/2003
• Status: Active Grant

• Current Assignee: Molecular Devices
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Katzlinger, Michael, Alaruri, Sami D., Atzler, Joseph
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US10/732,797
• Publication Number:
  US 20050122521A1
• Time in Patent Office: 1,022 Days
• Field of Search: None
• US Class Current: 356/432
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

An apparatus for analyzing a plurality of samples in sample sites on a substrate, the apparatus having an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used; a frame for supporting the substrate; a detector configured to detect light; an optical relay configured to transmit light from at least one of the plurality of light sources to a sample site and from the sample site to the detector; and a support structure for supporting the frame, the detector and the optical relay.

Claims

1. An apparatus for analyzing a plurality of samples in sample sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one of the light sources to one of the plurality of sample sites and from the sample site to the detector; and

e) a support structure for supporting the moveable support, the frame, the detector and the optical relay;

wherein the moveable support is moveable independently of the optical relay.

2. The apparatus of claim 1 further comprising a selection switch coupled to the moveable support.

7. The apparatus of claim 1 wherein the array of light sources further comprises at least one LED lamp.

8. The apparatus of claim 1 wherein the array of light sources further comprises a plurality of LED lamps, each of the plurality of LED lamps emitting light at a wavelength different than the others of the plurality of LED lamps.

9. The apparatus of claim 1 wherein the array of light sources further comprises:

an LED lamp having a wavelength range from about 450 nm to about 705 nm; and

an LED lamp having a wavelength centered at about one of 370, 430, 470, 590, 620, 910 and 960 nm.

18. A method for analyzing a sample, comprising:

selecting the apparatus of claim 1;

placing a sample at the sample site on the substrate;

selecting a light source; and

detecting emission light from the sample.

3. An apparatus for analyzing a plurality of samples in samples sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one of the light sources to one of the plurality of sample sites and from the sample site to the detector; and

e) a support structure for supporting the frame, the detector and the optical relay;

wherein the frame is configured to move the substrate in a first direction so that the sample sites pass sequentially through an examination region.

4. An apparatus for analyzing a plurality of samples in samples sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one of the light sources to one of the plurality of sample sites and from the sample site to the detector; and

e) a support structure for supporting the frame, the detector and the optical relay;

wherein the moveable support is a rotatable wheel.

5. An apparatus for analyzing a plurality of samples in samples sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one of the light sources to one of the plurality of sample sites and from the sample site to the detector; and

e) a support structure for supporting the frame, the detector and the optical relay;

wherein the moveable support further comprises an aperture for the passage of light from an exterior light source not positioned on the moveable support.

6. The apparatus of claim 5 wherein the exterior light source is a deuterium lamp.

10. An apparatus for analyzing a plurality of samples in sample sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one light source to one of the sample sites on the substrate and from the sample site to the detector; and

e) a support structure for supporting the stage, the detector and the optical relay;

wherein the optical relay further comprises a beam splitter for splitting light from at least one of the array of light sources into two beams, a first beam being directed to an upper surface of the sample site, and a second beam directed to a lower surface of the sample site.

11. The apparatus of claim 10 wherein the second beam is directed to a lower surface of the sample site by a fiber optic cable.

12. The apparatus of claim 10 wherein the second beam is directed to a lower surface of the sample site by a liquid waveguide.

13. The apparatus of claim 10 wherein the optical relay further comprises a first shutter selectively moveable to prevent light from the beam splitter from reaching the upper surface of the sample site.

14. The apparatus of claim 13 wherein the optical relay further comprises a second shutter selectively moveable to prevent light from the beam splitter from reaching the lower surface of the sample site.

15. The apparatus of claim 14 wherein the first and second shutter further comprise anodized steel.

16. The apparatus of claim 10 wherein the optical relay further comprises at least one of a first shutter selectively moveable to prevent light from the beam splitter from reaching the upper surface of the sample site and a second shutter selectively moveable to prevent light from the beam splitter from reaching the lower surface of the sample site.

17. An apparatus for analyzing a plurality of samples in sample sites on a substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a moveable support so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one light source to one of the sample sites on the substrate and from the sample site to the detector; and

e) a support structure for supporting the stage, the detector and the optical relay;

wherein the optical relay further comprises;

a beam splitter for splitting light from at least one of the array of light sources into two beams, a first beam being directed to an upper surface of the sample site, and a second beam directed to a lower surface of the sample site; and

a means for blocking at least one of the first beam and the second beam.

20. A method for analyzing a sample, comprising:

selecting the apparatus of claim 17;

placing a sample at the sample site on the substrate;

selecting a light source; and

detecting emission light from the sample.

19. An apparatus for analyzing a plurality of samples in sample sites on substrate, the apparatus comprising:

a) an array of spaced apart light sources coupled to a rotatable wheel so that one or more of the light sources can be selectively used;

b) a frame for supporting the substrate;

c) a detector configured to detect light;

d) an optical relay configured to transmit light from at least one light source to one of the sample sites on the substrate and from the sample site to the detector, the optical relay further comprising;

a beam splitter for splitting light from at least one of the array of light sources two beams, a first beam being directed to an upper surface of the sample site, and a second beam directed to a lower surface of the sample site; and

a first shutter selectively moveable to block the first beam; and

a second shutter selectively moveable to block the second beam; and

e) a support structure for supporting the stage, the detector and the optical relay; and

wherein at least one of the light sources is an LED.