Hydrodynamic focusing devices

• US 7,115,230 B2
• Filed: 06/26/2003
• Issued: 10/03/2006
• Est. Priority Date: 06/26/2003
• Status: Expired due to Fees

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Berlin, Andrew, Sundararajan, Narayanan
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US10/609,227
• Publication Number:
  US 20040266022A1
• Time in Patent Office: 1,195 Days
• Field of Search: 422/100, 422/103, 436/63, 137/89, 137/98
• US Class Current: 422/502
• CPC Class Codes: B01F 33/3011 using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A micro-fluidic device containing a micro-fluidic inlet channel to convey a process flow, a plurality of micro-fluidic focusing channels to each convey one of a plurality of focusing flows, a focusing manifold coupled with the inlet channel at an inlet port thereof and with the plurality of focusing channels at a plurality of focusing channel ports thereof to focus the process flow by contacting and hydrodynamically impacting at least three sides of the process flow with the focusing flows, and a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the combined focused process flow and focusing flow from the focusing manifold.

Claims

1. A device comprising:

a micro-fluidic inlet channel to convey a process flow;

a plurality of micro-fluidic focusing channels to each convey one of a plurality of focusing flows, wherein the plurality of focusing channels include a first channel on a first side of the inlet channel, a second channel on a second side of the inlet channel, a third upper channel over the inlet channel, and a fourth lower channel under the inlet channel, the first and second focusing channels being planar with the inlet channel and the third and fourth focusing channels being non-planar with the inlet channel;

a focusing manifold coupled with the inlet channel at an inlet port, coupled with the first channel at a first focusing channel port on a first side of the inlet channel, coupled with the second channel at a second focusing channel port on a second side of the inlet channel, coupled with the third channel at a third upper focusing channel port over the inlet channel on the first side of the inlet channel, and coupled with the fourth channel at a fourth lower focusing channel port under the inlet channel on the second side of the inlet channel, wherein the third upper focusing channel and the fourth lower focusing channel approach the focusing manifold from opposite sides thereof and are angled relative to the inlet channel with substantially equal angles, an additional upper focusing channel over the inlet channel on the second side of the inlet channel, and an additional lower focusing channel under the inlet channel on the first side of the inlet channel, wherein the additional upper focusing channel and the third upper focusing channel approach the focusing manifold from opposite sides thereof and are angled relative to the inlet channel with substantially equal angles and the additional lower focusing channel and the fourth lower focusing channel approach the focusing manifold from opposite sides thereof and are angled relative to the inlet, the focusing manifold to focus the process flow by contacting four sides of the process flow with the plurality of focusing flows; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the focused process flow and focusing flows from the focusing manifold.

2. The device of claim 1:

wherein the first and the second focusing channels are angled relative to the inlet channel with sub-orthogonal angles;

wherein the third and the fourth focusing channels approach the focusing manifold from a same side thereof as the inlet channel;

wherein the third focusing channel is substantially vertically aligned over the inlet channel and the fourth focusing channel is substantially vertically aligned under the inlet channel;

wherein the focusing manifold comprises a shape of an upright cylinder;

wherein the focusing manifold comprises an upper spacing volume between an upper focusing channel port and the inlet port; and

wherein the void volume is in a range between 0.0005 mm³ to 0.01 mm³.

3. A device comprising:

a micro-fluidic inlet channel to convey a process flow;

four micro-fluidic focusing channels to each convey one of a plurality of focusing flows, wherein the four micro-fluidic focusing channels comprise a first micro-fluidic focusing channel on a first side of the inlet channel, a second micro-fluidic focusing channel on a second side of the inlet channel, a third upper micro-fluidic focusing channel over the inlet channel, and a fourth lower micro-fluidic focusing channel under the inlet channel, the first and second focusing channels being planar with the inlet channel and the third and fourth focusing channels being non-planar with the inlet channel;

a focusing manifold coupled with the inlet channel at an inlet port and with the four focusing channels at a plurality of focusing channel ports to focus the process flow by contacting four sides of the process flow with the plurality of focusing flows; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the combined focused process flow and focusing flow from the focusing manifold.

4. The device of claim 3, wherein the plurality of focusing channel ports comprise a first focusing channel port on a first side of the inlet channel, a second focusing channel port on a second side of the inlet channel, a third upper focusing channel port over the inlet channel, and a fourth lower focusing channel port under the inlet channel.

5. The device of claim 3, wherein the third and the fourth focusing channels approach the focusing manifold from a same side thereof as the inlet channel.

6. The device of claim 5, wherein the third focusing channel is substantially vertically aligned over the inlet channel and the fourth focusing channel is substantially vertically aligned under the inlet channel.

7. The device of claim 3, wherein the focusing manifold comprises a shape of an upright cylinder, and wherein the void volume is in a range between 0.005 mm³ to 0.01 mm³.

8. The device of claim 3, wherein the focusing manifold comprises an upper spacing volume between an upper focusing channel port and the inlet port.

13. The device of claim 3, wherein the third upper focusing channel is over the inlet channel on the first side of the inlet channel, and the fourth lower focusing channel is under the inlet channel on the second side of the inlet channel.

14. The device of claim 13, wherein the third upper focusing channel and the fourth lower focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

15. The device of claim 14, wherein the substantially equal angles comprise orthogonal angles.

18. The device of claim 3, wherein the focusing manifold comprises a lower spacing volume between a lower focusing channel port and the inlet port.

19. The device of claim 3, wherein the first focusing channel and the second focusing channel are angled at substantially equal angles relative to the inlet channel.

20. The device of claim 19, wherein the angle is between 5 degrees and 90 degrees.

22. The device of claim 19, wherein the fifth lower focusing channel and the sixth lower focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

9. A device comprising:

a micro-fluidic inlet channel to convey a process flow;

six micro-fluidic focusing channels to each convey one of a plurality of focusing flows, wherein the six micro-fluidic focusing channels comprise a first focusing channel on a first side of the inlet channel, a second focusing channel on a second side of the inlet channel, the first and second focusing channels being planar with the inlet channel, a third upper focusing channel over the inlet channel on the first side, a fourth upper focusing channel over the inlet channel on the second side, a fifth lower focusing channel under the inlet channel on the first side, and a sixth lower focusing channel under the inlet channel on the second side;

a focusing manifold coupled with the inlet channel at an inlet port and with the six focusing channels at a plurality of focusing channel ports to focus the process flow by contacting at least four sides of the process flow with the plurality of focusing flows; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the combined focused process flow and focusing flow from the focusing manifold.

10. The device of claim 9, wherein the third upper focusing channel and the fourth upper focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

11. The device of claim 10, wherein the substantially equal angles comprise orthogonal angles.

12. The device of claim 9, wherein the third upper focusing channel is substantially vertically aligned over the fifth lower focusing channel and the fourth upper focusing channel is substantially vertically aligned over the sixth lower focusing channel.

21. The device of claim 9, wherein the third upper focusing channel and the fourth upper focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

23. The device of claim 9, wherein the focusing manifold comprises a shape of an upright cylinder, and wherein the void volume is in a range between 0.005 mm³ to 0.01 mm³.

24. The device of claim 9, wherein the focusing manifold comprises an upper spacing volume between an upper focusing channel port and the inlet port.

25. The device of claim 9, wherein the focusing manifold comprises a lower spacing volume between a lower focusing channel port and the inlet port.

26. The device of claim 9 wherein the plurality of focusing channel ports comprise a first focusing channel port on a first side of the inlet channel, a second focusing channel port on a second side of the inlet channel, a third upper focusing channel port over the inlet channel on the first side, a fourth upper focusing channel port over the inlet channel on the second side, a fifth lower focusing channel port under the inlet channel on the first side, and a sixth lower focusing channel port under the inlet channel on the second side.

27. The device of claim 9 wherein the a micro-fluidic outlet channel is on an opposite side of the focusing manifold as the inlet channel.

28. The device of claim 9, wherein the first focusing channel and the second focusing channel are angled at substantially equal angles relative to the inlet channel.

29. The device of claim 28, wherein the angle is between 5 degrees and 90 degrees.

16. A device comprising:

a micro-fluidic inlet channel to convey a process flow;

at least four micro-fluidic focusing channels to each convey one of a plurality of focusing flows, wherein the at least four micro-fluidic focusing channels include first and second focusing channels coplanar with the inlet channel, and third and fourth focusing channels not coplanar with the inlet channel, wherein the fourth micro-fluidic focusing channel is under the micro-fluidic inlet channel;

a focusing manifold coupled with the inlet channel at an inlet port, coupled with the at least four micro-fluidic focusing channels at a plurality of focusing channel ports; and

a micro-fluidic outlet channel coupled with the focusing manifold at an outlet channel port to convey the focused process flow and focusing flows from the focusing manifold.

17. The device of claim 16, wherein the third micro-fluidic focusing-channel is over the micro-fluidic inlet channel.

30. The device of claim 16, wherein the plurality of focusing channel ports comprise a first focusing channel port on a first side of the inlet channel, a second focusing channel port on a second side of the inlet channel, a third upper focusing channel port over the inlet channel, and a fourth lower focusing channel port under the inlet channel.

31. The device of claim 16, wherein the first focusing channel is on a first side of the inlet channel, the second focusing is on a second side of the inlet channel, the third focusing channel is over the inlet channel on the first side and the fourth upper focusing channel over the inlet channel on the second side.

32. The device of claim 31, wherein the third focusing channel and the fourth focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

33. The device of claim 16, further comprising fifth and sixth focusing channels not coplanar with the inlet channel, wherein the third and fourth focusing channels are above the inlet channel and the fifth and sixth focusing channels are below the inlet channel.

34. The device of claim 33, wherein the third upper focusing channel and the fourth upper focusing channel are on opposite sides of the focusing manifold and are angled at substantially equal angles relative to the inlet channel.

35. The device of claim 33, wherein the third upper focusing channel is substantially vertically aligned over the fifth lower focusing channel and the fourth upper focusing channel is substantially vertically aligned over the sixth lower focusing channel.