Multiple laminar flow-based particle and cellular separation with laser steering

• US 7,118,676 B2
• Filed: 09/03/2004
• Issued: 10/10/2006
• Est. Priority Date: 09/04/2003
• Status: Expired due to Fees

• Current Assignee: Premium Genetics Ltd. (Genus PLC)
• Original Assignee: Arryx, Inc. (Haemonetics Corporation)
• Inventors: Anderson, Amy, Rosenbaum, Neil Harris, Shireman, Jessica, Plewa, Joseph, Mueth, Daniel, Gruber, Lewis
• Primary Examiner(s): NGUYEN, KIET TUAN
• Application Number:
  US10/934,597
• Publication Number:
  US 20050121604A1
• Time in Patent Office: 767 Days
• Field of Search: 250/251, 210/732, 210/800, 210/802, 210/927, 435/173.1, 435/72, 435/82.05, 435/101, 436/177
• US Class Current: 210/732
• CPC Class Codes: A61M 1/36 Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
• CPC Class Codes: A61M 1/3603 in the same direction
• CPC Class Codes: A61M 1/3681 by irradiation
• CPC Class Codes: A61M 1/3693 using separation based on different densities of components, e.g. centrifuging
• CPC Class Codes: A61M 1/38 Removing constituents from donor blood and storing or returning remainder to body , e.g. for transfusion
• CPC Class Codes: A61M 2206/11 Laminar flow
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B03C 1/28 Magnetic plugs and dipsticks
• CPC Class Codes: B03C 2201/02 Electro-statically separating liquids from liquids
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 5/005 Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength B03C5/02 takes precedence; electrophoresis B01D57/02
• CPC Class Codes: G01N 30/0005 Field flow fractionation
• CPC Class Codes: G03H 1/08 Synthesising holograms, i.e. holograms synthesized from objects or objects from hologramsusing electric digital computers G06F; G06T
• CPC Class Codes: G03H 1/2294 Addressing the hologram to an active spatial light modulator
• CPC Class Codes: G03H 2001/0077 for optical manipulation, e.g. holographic optical tweezers [HOT]
• CPC Class Codes: G03H 2001/085 Kinoform, i.e. phase only encoding wherein the computed field is processed into a distribution of phase differences
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

Claims

1. A system for separation of a plurality of components from a fluid mixture, the system comprising:

a flow plate having a plurality of inlets for a first flow and a second flow and having a plurality of outlets for the first flow and the second flow, the flow plate further having at least one separation region in which the first flow and the second flow are substantially laminar;

a plurality of pumps to control the flow rates of the first flow and the second flow;

a temperature control to control the temperature of the first flow and the second flow; and

a computer adapted to provide user input for control of the separation of the plurality of components from the fluid mixture in the separation region.