Modified ADAMTS4 molecules and method of use thereof
First Claim
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1. An isolated, modified, human ADAMTS4 protein comprising(a) an ADAMTS4 catalytic domain wherein the glutamate at the position corresponding to position 362 of SEQ ID NO:
- 1 is substituted with a glutamine, and(b) a disintegrin domain.
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Abstract
The present invention relates to modified ADAMTS4 proteins having improved stability comparing to the corresponding native, unmodified proteins. The modified ADAMTS4 proteins can be expressed and isolated in large quantities, thus allowing further characterization of the proteins, such as crystallographic and enzyme kinetic studies. The purified, stable proteins would also facilitate the production of anti-ADAMTS antibodies and the development of inhibitors to ADAMTS enzymes.
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14 Claims
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1. An isolated, modified, human ADAMTS4 protein comprising
(a) an ADAMTS4 catalytic domain wherein the glutamate at the position corresponding to position 362 of SEQ ID NO: - 1 is substituted with a glutamine, and
(b) a disintegrin domain. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- 1 is substituted with a glutamine, and
Specification