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Microfluidic device and methods of using same

  • US 7,118,910 B2
  • Filed: 11/27/2002
  • Issued: 10/10/2006
  • Est. Priority Date: 11/30/2001
  • Status: Expired due to Fees
First Claim
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1. A microfluidic device, comprising:

  • (a) a first plurality of flow channels formed in an elastomeric substrate;

    (b) a second plurality of flow channels formed in the elastomeric substrate that intersect the first plurality of flow channels to define an array of reaction sites, each reaction site located at an intersection of one of the first and second flow channels;

    (c) a plurality of isolation valves disposed along the first and second plurality of flow channels and spaced between the reaction sites that can be actuated to isolate a solution within each of the reaction sites from solutions at other reaction sites, wherein the isolation valves comprise one or more control channels that each overlay and intersect one or more of the flow channels; and

    (d) means for simultaneously actuating the valves for isolating the reaction sites from each other.

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