Self-contained microfluidic biochip and apparatus

• US 7,122,153 B2
• Filed: 01/08/2003
• Issued: 10/17/2006
• Est. Priority Date: 01/08/2003
• Status: Expired due to Fees

• Current Assignee: Winston Z. Ho
• Original Assignee: Winston Z. Ho
• Inventors: Ho, Winston Z.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Turk, Neil
• Application Number:
  US10/338,451
• Publication Number:
  US 20040132218A1
• Time in Patent Office: 1,378 Days
• Field of Search: 422/55, 422/57, 422/58, 436/6, 436/5
• US Class Current: 422/417
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

A biochip and apparatus is disclosed for performing biological assays in a self-contained microfluidic platform. The disposable biochip for multi-step reactions comprises a body structure with a plurality of reagent cavities and reaction wells connected via microfluidic channels; the reagent cavities with reagent sealing means for storing a plurality of reagents; the reagent sealing means being breakable and allowing a sequence of reagents to be released into microfluidic channel and reaction well; and the reaction well allowing multi-step reactions to occur by sequentially removing away the residual reagents. The analysis apparatus can rapidly, automatically, sensitively, and simultaneously detect and identify multiple analytes or multiple samples in a very small quantity.

Claims

1. A self-contained disposable microfluidic biochip for performing multi-step reactions comprising:

a multi-layered body structure comprising plurality of layers, including a first layer defining a plurality of reagent cavities and at least second layer defining a plurality of reaction wells connected via microfluidic channels;

said reagent cavities respectively storing a plurality of reagents, wherein a seal is provided between the reagent cavities in the first layer and the microfluidic channels in the second layer;

said seal comprising a separate thin film located at the bottom of each reagent cavity to prevent escape of fluids through said microchannels; and

said thin film being breakable and allowing said reagents to be released sequentially into at least one of said microfluidic channels and said reaction wells one at a time;

an integrated micro cap assembly located within each said reagent cavity comprising a pin for puncturing said thin film and a stopper slidable in each said reagent cavity to press a respective one of said reagents into said microfluidic channels;

said reaction wells, allowing sample input, and allowing said multi-step reactions to occur by removing away a sequence of said reagents.

2. The biochip defined in claim 1, wherein said body structure is formed by bonding multiple layers of plastic materials.

3. The biochip defined in claim 1, wherein said microfluidic channels have a dimension between 0.5 μ

m and 2 mm in cross section.

4. The biochip defined in claim 1, wherein one of said reagents is selected from a group consisting of buffer solutions, labeling substances, proteins, nucleic acids and chemicals.

5. The biochip defined in claim 1, wherein said reaction wells are facilitated with biological probes.

6. The biochip defined in claim 5, wherein one of said biological probes is selected from a group consisting of proteins, nucleic acids, receptors, and cells.

7. An analysis apparatus comprising a biochip defined in claim 1, and further comprising:

(a) a microactuator, positioned relative to each of said reagent cavities, to apply downward pressure to said micro cap assembly in each of said reagent cavities;

(b) a vacuum line positioned relative to at least one reaction well to remove residual reagent out of said reaction well; and

(c) a detector located either above or below said reaction well for detecting optical signal generated in said reaction well.

8. The analysis apparatus defined in claim 7, further comprising a microprocessor for controlling said microactuator, said vacuum line, and said detector.

9. The analysis apparatus defined in claim 7, wherein said body structure is formed by bonding multiple layers of plastic materials.

10. The analysis apparatus defined in claim 7, wherein said microfluidic channels have a dimension between 0.5 μ

m and 2 mm in cross section.

11. The analysis apparatus defined in claim 7, wherein one of said reagents is selected from a group consisting of buffer solutions, labeling substances, proteins, nucleic acids and chemicals.

12. The analysis apparatus defined in claim 7, wherein said reaction wells are facilitated with biological probes.

13. The analysis apparatus defined in claim 12, wherein one of said biological probes is selected from a group consisting of proteins, nucleic acids, receptors, and cells.

14. The biochip defined in claim 1, wherein the second layer comprises a first sub-layer defining the microchannels and a second sub-layer defining the reactions wells.

15. A method of performing multi-step reactions comprising:

providing a biochip as defined in claim 1;

providing a sample in at least one reaction well;

activating the microcap assembly at a selected number of reagent cavities in a desired sequence, to deliver selected reagents into said at least one reaction well in a desired sequence; and

reacting said sample and said selected reagents in said at least one reaction well.

16. A self contained disposable microfluidic biochip for performing multi-step reactions, comprising:

a body comprising a layered structure, including;

a first layer that comprises a plurality of reagent cavities each containing a reagent,

a second layer that comprises at least one reaction well in fluid communication with the plurality of reagent cavities, and

a separate layer of a sealing material disposed between the first layer and the second layer,which retains the reagent in each reagent cavity, and which is breakable at each regent cavity to permit flow of reagent into the reaction well,

wherein the first layer, the sealing layer and the second layer are bonded together to form the layered structure; and

a microcap assembly slidably received in each resent cavity, the microcap assembly comprising a stopper and a pin extending from the stopper towards the layer of sealing material, wherein the pin punctures the layer of sealing material when the stopper is pressed towards the layer of sealing material.

17. The biochip defined in claim 16, wherein the seal layer extends to the plurality of reagent cavities.

18. The biochip defined in claim 16, wherein the second layer further defines a network of microchannels extending from the reaction well to the layer of sealing material below the respective reagent cavities.