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Method for enhancing amplification of a target nucleic acid sequence

  • US 7,122,316 B2
  • Filed: 02/27/2003
  • Issued: 10/17/2006
  • Est. Priority Date: 07/31/1998
  • Status: Expired due to Fees
First Claim
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1. A method useful for amplifying a target nucleic acid sequence comprising the steps of:

  • a) producing a mixture comprising an RNA polymerase and a reversible inhibitor of said RNA polymerase, wherein said reversible inhibitor does not hybridize to a target nucleic acid comprising said target nucleic acid sequence under transcription-associated amplification conditions, and wherein said mixture does not contain said target nucleic acid;

    b) combining said mixture and a sample suspected of containing said target nucleic acid; and

    c) exposing the combination of step b) to said amplification conditions, such that said target nucleic acid sequence, if present in said sample, is amplified.

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