Carriers having biological substance

• US 7,122,378 B1
• Filed: 03/06/2000
• Issued: 10/17/2006
• Est. Priority Date: 03/05/1999
• Status: Expired due to Fees

• Current Assignee: Mitsubishi Chemical Corporation (Mitsubishi Chemical Group Corp.)
• Original Assignee: Mitsubishi Rayon Company Limited (Mitsubishi Chemical Holdings Corporation)
• Inventors: Fujii, Wataru, Sumi, Toshinori, Ishimaru, Teruta, Oogami, Nobuko, Ito, Chiho, Maehara, Osamu, Makino, Takayuki, Uragaki, Toshitaka, Takahashi, Atsushi, Akita, Takashi, Murase, Kei, Yu, Fujio, Watanabe, Fumiaki, Miyauchi, Haruko, Morishita, Takeharu, Ikeda, Tadanobu
• Primary Examiner(s): Sisson, Bradley L.
• Application Number:
  US09/914,782
• Time in Patent Office: 2,416 Days
• Field of Search: 435/6, 435/287.1, 435/287.2, 436/94, 536/23.1
• US Class Current: 436/94
• CPC Class Codes: D06M 15/13 Alginic acid or derivatives thereof
• CPC Class Codes: D06M 15/15 Proteins or derivatives thereof
• CPC Class Codes: D06M 15/263 of unsaturated carboxylic acids; Salts or esters thereof
• CPC Class Codes: D06M 15/285 of unsaturated carboxylic acid amides or imides
• CPC Class Codes: D06M 15/333 of vinyl acetate; Polyvinylalcohol
• CPC Class Codes: D06M 15/3562 containing nitrogen
• CPC Class Codes: D06M 15/53 Polyethers polyacetals D06M15/39
• CPC Class Codes: D06M 16/00 Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
• CPC Class Codes: D06M 23/00 Treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, characterised by the process
• CPC Class Codes: D06M 23/02 Processes in which the treating agent is releasably affixed or incorporated into a dispensing means
• CPC Class Codes: Y10T 156/1052 with cutting, punching, tearing or severing
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

By the present invention, there is provided a fiber having nucleic acid immobilized thereon, an alignment of fibers having nucleic acid immobilized thereon, and a slice thereof.

Claims

1. A method for determining the two-dimensional coordinates of sequential cross-sectional slices of a fiber bundle which may be used to form a chip or a micro-array comprising:

(a) producing a series of sequential cross-sectional slices of a bundle of adhered linearly-aligned fibers, wherein each sequential slice comprises multiple fiber units that are bound or immobilized to each other, and each bound or immobilized fiber unit is produced by the cross-sectional slicing of the individual fibers in the bundle,

wherein said fiber bundle comprises fibers containing one or more immobilized biological substance(s) and at least two coordinate reference points,

(b) determining the two-dimensional coordinates of each fiber unit within a fiber alignment slice S(h) using the coordinates of the fiber units formed by said two or more coordinate reference points;

(c) determining the two-dimensional coordinates of each fiber unit corresponding to those in fiber alignment slice S(h) which are in an adjacent or sequential fiber alignment slice S(i) of said fiber bundle, based on the coordinate data obtained for fiber alignment slice S(h) in step (b) and based on the positions of the coordinate reference points in the adjacent or sequential fiber alignment slice; and

(d) repeating steps (b) and (c) to determine the two-dimensional coordinates of fiber units in one or more successive or adjacent fiber alignment slices of said fiber bundle.

2. The method of claim 1, wherein, in step (b), the two-dimensional coordinates of each fiber unit in slice S(h) are first determined relative to an XY plane, and the resulting values are then translated into a coordinate system based on the coordinate reference points in said slice S(h), to form translated coordinates for each fiber in slice S(h).

3. The method of claim 1, wherein said fibers are selected from the group consisting of hollow fibers incorporating an immobilized biological substance, porous fibers incorporating an immobilized biological substance, and porous hollow fibers incorporating an immobilized biological substance, wherein the biological substance is directly immobilized on the fiber, in the fiber, or both on and in the fiber.

4. The method of claim 1, wherein said fibers are fibers retaining a gel which incorporates one or more immobilized biological substance(s), whereby the biological substance is immobilized on the fiber, in the fiber, or both on and in the fiber.

5. The method of claim 2, wherein, in step (c), the two-dimensional coordinates of each fiber unit in slice S(i) are first determined relative to an XY plane, using the corresponding translated coordinates for each fiber in slice S(h), and the resulting values are then translated into a coordinate system based on the coordinate reference points in said slice S(i).

6. The method of claim 3, wherein the one or more biological substance(s) is one selected from a group consisting of:

(a) nucleic acid, amino acid, sugar or lipid;

(b) a polymer consisting of one or more kinds of ingredients from the substances stated in (a) above; and

(c) a substance interacting with substances stated in (a) or (b) above.

7. The method of claim 4, wherein said fibers are selected from the group consisting of solid fibers, hollow fibers, porous fibers and hollow porous fibers.

8. The method of claim 6, wherein the one or more biological substance(s) is nucleic acid.

9. The method of claim 4, wherein said fibers also have a pigment retained on the fiber, in the fiber or both on and in the fiber, by means of the gel.

10. The method of claim 4, wherein said fiber bundles contain 100 or more individual fibers.

11. The method of claim 4, wherein said fiber bundles contain 1000 to 10,000,000 individual fibers.

12. The method of claim 4, wherein said fiber bundles have a fiber density ranging from 100 to 1,000,000 fibers per cm².

13. The method of claim 4, wherein the thickness of the fibers is 1 mm or less.

14. The method of claim 5, wherein the coordinate reference points correspond to chosen fiber units in said bundle.

15. The method of claim 5, wherein the coordinate reference points correspond to lines drawn on the side(s) of the fiber alignment.

16. The method of claim 5, wherein the coordinate reference points correspond to slots on the side(s) of the fiber alignments.

17. The method of claim 5, wherein said bundle contains 2 coordinate reference points per 1,000 fiber units.

18. The method of claim 7, wherein said fibers are solid fibers, and wherein the gel incorporating an immobilized biological substance is retained on a surface of the fibers.

19. The method of claim 7, wherein said fibers are hollow fibers, and wherein the gel incorporating an immobilized biological substance is retained in a hollow part of the fibers.

20. The method of claim 7, wherein said fibers are porous fibers, and wherein the gel incorporating an immobilized biological substance is retained in the pore(s) of the fibers.

21. The method of claim 7, wherein said fibers are porous hollow fibers, and wherein the gel incorporating an immobilized biological substance is retained in a hollow part and the pore(s) of the fibers.

22. The method of claim 4, wherein the one or more biological substance(s) is selected from a group consisting of:

(a) nucleic acid, amino acid, sugar or lipid;

(b) a polymer consisting of one or more kinds of ingredients from the substances stated in (a) above; and

(c) a substance interacting with substances stated in (a) or (b) above.

23. The method of claim 22, wherein the biological substance is nucleic acid.

24. A method for determining the two-dimensional coordinates of sequential cross-sectional slices of a fiber bundle which may be used to form a chip or a micro-array comprising:

(a) producing a series of sequential cross-sectional slices of a bundle of adhered linearly-aligned fibers, wherein each sequential slice comprises multiple fiber units that are bound or immobilized to each other, and each bound or immobilized fiber unit is produced by the cross-sectional slicing of the individual fibers in the bundle,

wherein said fiber bundle comprises fibers containing one or more immobilized biological substance(s), and

the bundle contains two or more coordinate reference points;

(b) determining the two-dimensional coordinates of each fiber unit within a fiber alignment slice S(h) using the coordinate reference points;

(c) determining the two-dimensional coordinates of each fiber unit corresponding to those in fiber alignment slice S(h) which are in an adjacent or sequential fiber alignment slice S(i) of said fiber bundle, based on the coordinate data obtained for fiber alignment slice S(h) in step (b) and based on the positions of the coordinate reference points in the adjacent or sequential fiber alignment slice; and

(d) repeating steps (b) and (c) to determine the two-dimensional coordinates of fiber units in one or more successive or adjacent fiber alignment slices of said fiber bundle.