Selective affinity material, preparation thereof by molecular imprinting, and use of the same

• US 7,122,381 B2
• Filed: 09/04/1992
• Issued: 10/17/2006
• Est. Priority Date: 09/06/1991
• Status: Expired due to Fees

• Current Assignee: Klaus Mosbach, Magnus Glad, Maria Kempe
• Original Assignee: Klaus Mosbach, Magnus Glad, Maria Kempe
• Inventors: Kempe, Maria, Mosbach, Klaus, Glad, Magnus
• Primary Examiner(s): Mannifield, Nita
• Assistant Examiner(s): Hines, J.
• Application Number:
  US08/199,300
• Publication Number:
  US 20030049870A1
• Time in Patent Office: 5,156 Days
• Field of Search: 435/4, 435/6, 435/7.1, 435/174, 435/181, 435/283.1, 435/287.1, 435/287.6, 435/287.7, 435/287.8, 436/518, 436/525, 436/527, 436/529, 436/530, 436/531
• US Class Current: 436/518
• CPC Class Codes: B01D 15/3852 using imprinted phases or molecular recognition; using imprinted phases
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/281 Sorbents specially adapted for preparative, analytical or investigative chromatography
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: B01J 20/3092 Packing of a container, e.g. packing a cartridge or column of chromatography columns B01D15/206
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3208 Polymeric carriers, supports or substrates
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3244 Non-macromolecular compounds
• CPC Class Codes: B01J 20/3257 the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such
• CPC Class Codes: B01J 20/3285 Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template

Abstract

A selective adsorption material made by the process comprising: a) non-cavalently or reversibly-covalently binding a print molecule to at least two styrene, acrylate or silica monomers, each of which monomers brinds to said print molecule by means of a different functional group; b) immobilizing said bound monomers by being polymerized to each other in the presence of an effective amount of cross-linker; and c) removing said print molecule from said polymer by extraction to leave a cavity in said polymer which is stereo-tailored to a biological molecule of interest; and wherein said print molecule is the biological molecule of interest.

Claims

1. A process for producing a molecularly imprinted adsorption material, comprising the steps of:

forming non-covalent or reversible-covalent bonds between at least two ligands and a protein print molecule, said at least two ligands becoming at least two bound ligands, each one of said at least two ligands comprising at least two different functional groups, having at least one of said at least two different functional groups being an imidazole-metal, an iminodiacetic acid-metal, a carboxyl, an amine, a thiol, or an aldehyde and which is reversibly bound to said print molecule;

immobilizing said at least two bound ligands to a matrix by covalently binding at least one of said at least two different functional groups to said matrix;

extracting said protein print molecule from said immobilized at least two bound ligands, said extracting of said protein print molecule forming a stereo-tailored zone;

producing a molecularly imprinted adsorption material.

2. The molecularly imprinted adsorption material produced by the process of claim 1, having said functional group comprising an imidazole-metal or an iminodiacetic acid-metal and said functional group being reversibly bound to a protein print molecule.

3. The molecularly imprinted adsorption material produced by the process of claim 1, in which said ligands are monomers and in which said immobilizing comprises a further step of polymerizing said monomers.

4. The molecularly imprinted adsorption material produced by the process of claim 1, having said stereo-tailored zone configured to an enzyme or glycoprotein.

5. The molecularly imprinted adsorption material produced by the process of claim 1, having said matrix formed through the polymerization of said different functional groups into a cross-linked polymer.

6. The process according to claim 1, further comprising the step of:

forming said matrix through the polymerization of said different functional groups into a cross-linked polymer.

7. The process according to claim 1, further comprising the step of:

binding at least one of said at least two different functional groups to an already existing matrix.

8. A molecularly imprinted adsorption material for adsorbing protein molecules comprising:

a matrix,

at least two ligands covalently bound to the surface of said matrix by directed immobilization forming at least two immobilized ligands, in which each of said at least two ligands include at least two different functional groups, in which at least one of said at least two different functional groups is an imidazole-metal or an iminodiacetic acid-metal,

a molecularly imprinted stereo-tailored zone.

9. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a silica, a glass, or mixtures thereof.

10. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a methacrylate-silica material.

11. The molecularly imprinted adsorption material of claim 8, having said matrix comprising an acrylate or a methacrylate surface bound reactive group.

12. The molecularly imprinted adsorption material of claim 8, having the adsorption material stereo-tailored to selectively adsorb a protein.

13. The molecularly imprinted adsorption material of claim 8, having the adsorption material stereo-tailored to selectively adsorb an enzyme.

14. The molecularly imprinted adsorption material as claimed in claim 8, having said matrix comprising a silica, a silane and a derivatized vinyl monomer.

15. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a biological polymer, a synthetic polymer, or mixtures thereof.

16. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a monomer.

17. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a vinyl surface bound reactive group.

18. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a hydroxyl surface bound reactive group.

19. The molecularly imprinted adsorption material of claim 8, having said matrix comprising a silanol surface-bound reactive group.

20. The molecularly imprinted adsorption material of claim 8, having the adsorption material stereo-tailored to selectively adsorb a nucleic acid.

21. The molecularly imprinted adsorption material of claim 8, having the adsorption material stereo-tailored to selectively adsorb a polysaccharide.

22. The molecularly imprinted adsorption material of claim 8, having the adsorption material stereo-tailored to selectively adsorb a glycoprotein.