Process for aligning macromolecules by passage of a meniscus and applications

• US 7,122,647 B2
• Filed: 02/13/2003
• Issued: 10/17/2006
• Est. Priority Date: 02/11/1994
• Status: Expired due to Fees

• Current Assignee: Centre National De La Recherche Scientifique, Institut Pasteur
• Original Assignee: Centre National De La Recherche Scientifique, Institut Pasteur
• Inventors: Heslot, François, Bensimon, Aaron, Bensimon, David
• Primary Examiner(s): FREDMAN, JEFFREY NORMAN
• Application Number:
  US10/365,693
• Publication Number:
  US 20030175779A1
• Time in Patent Office: 1,342 Days
• Field of Search: None
• US Class Current: 536/23.1
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00628 Ionic
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 33/6803 : General methods of protein analysis not limited to specific proteins or families of proteins
• CPC Class Codes: Y10S 435/969 : Multiple layering of reactants
• CPC Class Codes: Y10S 435/97 : Test strip or test slide
• CPC Class Codes: Y10S 530/81 : Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
• CPC Class Codes: Y10S 530/811 : Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/145555 : Hetero-N

Abstract

A product includes a surface on which a coupled complex is aligned, adherent, and stationary, obtained by a process that includes providing a support having a surface with a molecule with biological activity attached, contacting the surface with a sample containing a macromolecule to couple the macromolecule to the molecule on the surface, passing a meniscus over the coupled complex to align the coupled complex, and detecting the macromolecule in the coupled complex. The product is alternatively obtained by passing a meniscus over the molecule with biological activity prior to contacting the surface with a sample containing a macromolecule to couple the macromolecule to the molecule on the surface.

Claims

1. A product obtained by a process for detecting a macromolecule in a sample, wherein the process comprises:

(a) providing a support having a surface, wherein the surface comprises a molecule with biological activity attached thereto and wherein the molecule with biological activity comprises biotin, a protein, a nucleic acid, a lipid, or a polysaccharide;

(b) contacting the sample with the surface to couple the macromolecule in the sample to the molecule with biological activity;

(c) passing a meniscus over the coupled complex from step (b) to align the coupled complex on the surface; and

(d) detecting, either directly or indirectly, the macromolecule in the coupled complex;

wherein the product comprises the surface on which the coupled complex is adherent and stationary.

2. A product obtained by a process for detecting a macromolecule in a sample, wherein the process comprises:

(a) providing a support having a surface, wherein the surface comprises a molecule with biological activity, wherein the molecule with biological activity has been aligned on the surface by passage of a meniscus over the molecule with biological activity, and wherein the molecule with biological activity comprises biotin, a protein, a nucleic acid, a lipid, or a polysaccharide;

(b) contacting the sample with the surface to couple the macromolecule in the sample to the molecule with biological activity; and

(c) detecting, either directly or indirectly, the macromolecule in the coupled complex from step (b);

wherein the product comprises the surface on which the coupled complex is aligned, adherent, and stationary.

3. The product obtained by a process for detecting a macromolecule in a sample, wherein the process comprises:

(a) providing a support having a surface;

(b) anchoring a molecule with biological activity to the surface, wherein the molecule with biological activity comprises biotin, a protein, a nucleic acid, a lipid, or a polysaccharide;

(c) contacting the sample with the surface to couple the macromolecule in the sample to the molecule with biological activity;

(d) passing a meniscus over the coupled complex from step (c) to align the coupled complex on the surface; and

(e) detecting, either directly or indirectly, the macromolecule in the coupled complex;

wherein the product comprises the surface on which the coupled complex is adherent and stationary.

4. The product obtained by a process for detecting a macromolecule in a sample, wherein the process comprises:

(a) providing a support having a surface;

(b) anchoring a molecule with biological activity to the surface, wherein the molecule with biological activity comprises biotin, a protein, a nucleic acid, a lipid, or a polysaccharide;

(c) passing a meniscus over the molecule with biological activity to align the molecule with biological activity on the surface;

(d) contacting the sample with the surface to couple the macromolecule in the sample to the molecule with biological activity; and

(e) detecting, either directly or indirectly, the macromolecule in the coupled complex from step (d);

wherein the product comprises the surface on which the coupled complex is aligned, adherent, and stationary.