Assay methods and systems
First Claim
1. A kit, comprising:
- a fluorescently labeled phosphorylatable compound which is capable of being phosphorylated to produce a fluorescently labeled phosphorylated product; and
a liquid reagent comprising a polymer having multivalent metal cations associated therewith, wherein the multivalent metal cations bind the polymer to the phosphrylated product.
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Accused Products
Abstract
Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.
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Citations
22 Claims
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1. A kit, comprising:
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a fluorescently labeled phosphorylatable compound which is capable of being phosphorylated to produce a fluorescently labeled phosphorylated product; and a liquid reagent comprising a polymer having multivalent metal cations associated therewith, wherein the multivalent metal cations bind the polymer to the phosphrylated product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A kit, comprising:
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a fluorescently labeled phosphorylatable compound which is capable of being phosphorylated to produce a fluorescently labeled phosphorylated product; and a liquid reagent comprising a molecule having multivalent metal cations associated therewith, wherein the metal cations bind the molecule to the phosphorylated product. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification