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Near infrared chemical imaging microscope

  • US 7,123,360 B2
  • Filed: 10/24/2005
  • Issued: 10/17/2006
  • Est. Priority Date: 10/13/2000
  • Status: Expired due to Term
First Claim
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1. A method for producing a volumetric image of a sample comprising the steps of:

  • A) illuminating an area of a sample using light in the near infrared wavelength radiation and the visible wavelength radiation so as to yield a near infrared wavelength image and a visible wavelength image of said sample;

    B) collecting a spectrum of near infrared wavelength radiation light transmitted, reflected, or emitted from said illuminated area of said sample and producing a collimated beam therefrom;

    C) selecting a near infrared wavelength image of said collimated beam with a near infrared imaging spectrometer;

    D) capturing said filtered near infrared wavelength image;

    E) processing the captured near infrared wavelength image and visible wavelength image;

    F) moving said sample relative to an objective;

    G) collecting near infrared wavelength and visible wavelength images of said sample relative to said objective in a plurality of focus depths using steps A–

    F; and

    H) processing said collected near infrared wavelength and visible wavelength images to reconstruct said volumetric image of said sample.

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