Systems and methods for detection of analytes in biological fluids
First Claim
1. A kit comprisinga first component comprising biotin linked, through a polyethylene glycol spacer, to albumin,a second component comprising a biotin-binding moiety conjugated to an antigen or hapten that binds an antimicrobial antibody to be detected in a sample, anda third component comprising the antigen or hapten conjugated to a label.
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Abstract
The invention provides a heterogeneous immunoassay for detection of antibodies and antigens based on specific antigen-antibody immune complex formation with multiple antigen-bearing conjugate components. The invention further provides means for optimizing the assay format for the detection of both low and high-affinity antibodies, and provides means for quantitative detection of both antibody and the corresponding antigen present in a sample.
27 Citations
13 Claims
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1. A kit comprising
a first component comprising biotin linked, through a polyethylene glycol spacer, to albumin, a second component comprising a biotin-binding moiety conjugated to an antigen or hapten that binds an antimicrobial antibody to be detected in a sample, and a third component comprising the antigen or hapten conjugated to a label.
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12. A kit comprising
a solid support to which is noncovalently adsorbed a first component comprising biotin linked, through a polyethylene glycol spacer, to albumin; -
a second component comprising a biotin-binding moiety conjugated to an antigen or hapten that binds an antimicrobial antibody to be detected in a sample; and a third component comprising the antigen or hapten conjugated to a label. - View Dependent Claims (13)
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Specification