Animal tissue with carbohydrate antigens compatible for human transplantation
First Claim
1. A transgenic pig whose genome comprises a homozygous inactivation of the α
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
1,2FT, operably linked to a promoter and expressed wherein cells, tissue, or organs of said pig exhibit reduced hyperacute rejection.
1 Assignment
0 Petitions
Accused Products
Abstract
This disclosure provides a system for generating animal tissue with carbohydrate antigens that are compatible for transplantation into human patients. The tissue is inactivated homozygously for expression of α(1,3)galactosyltransferase, and comprises a transgene for α(1,2)fucosyltransferase. As a result, cell-surface N-acetyl lactosamine is not converted to the Galα(1,3)Gal xenoantigen. Instead, it is converted to Fucα(1,2)Gal, which is H substance, a self-antigen in humans. The tissue may also contain A or B-transferase, which will cause H substance to be converted into other ABO blood group antigens for compatibility with patients of the same blood type. This invention improves transplant compatibility of the xenograft tissue by lessening the risk of reactions resulting from xenoantigen and unconverted N-acetyl lactosamine acceptor determinants.
-
Citations
15 Claims
-
1. A transgenic pig whose genome comprises a homozygous inactivation of the α
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
1,2FT, operably linked to a promoter and expressed wherein cells, tissue, or organs of said pig exhibit reduced hyperacute rejection. - View Dependent Claims (2, 3, 4)
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
-
5. An isolated porcine cell whose genome comprises a homozygous inactivation of the α
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
1,2FT, operably linked to a promoter and expressed wherein said cell exhibits reduced hyperacute rejection. - View Dependent Claims (6, 7, 8)
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
-
9. Isolated porcine tissue comprising a porcine cell whose genome comprises a homozygous inactivation of the α
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
1,2FT, operably linked to a promoter and expressed wherein said tissue exhibits reduced hyperacute rejection. - View Dependent Claims (10, 11, 12, 13, 14, 15)
- 1,3GT gene, and wherein said genome further comprises a DNA sequence encoding α
Specification