Multivalent antibody constructs
First Claim
1. A bispecific tetravalent homodimeric Fv antibody formed by two single-chain Fv monomers, each of said Fv monomers having at least four variable domains, whereinsaid four variable domains are VH-A, VL-A, VH-B and VL-B, wherein VH-A and VL-A are VH and VL domains of an antibody specific for antigen A, respectively, and VH-B and VL-B are VH and VL domains of an antibody specific for antigen B, respectively;
- VH-A is linked to VL-B by peptide linker 1, VL-B is linked to VH-B by peptide linker 2, VH-B is linked to VL-A by peptide linker 3; and
said peptide linker 1 and said peptide linker 3 are a peptide bond or have about 1 to about 10 amino acids; and
said peptide linker 2 has 3 to about 10 amino acids.
1 Assignment
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Accused Products
Abstract
The present invention relates to multivalent Fvantibody construct having at least four variable domains which are linked with each over via the peptide linkers 1, 2 and 3. The invention also concerns expression plasmids which code for such an Fvantibody construct and a method of producing the Fvantibody constructs as well as their use.
98 Citations
4 Claims
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1. A bispecific tetravalent homodimeric Fv antibody formed by two single-chain Fv monomers, each of said Fv monomers having at least four variable domains, wherein
said four variable domains are VH-A, VL-A, VH-B and VL-B, wherein VH-A and VL-A are VH and VL domains of an antibody specific for antigen A, respectively, and VH-B and VL-B are VH and VL domains of an antibody specific for antigen B, respectively; -
VH-A is linked to VL-B by peptide linker 1, VL-B is linked to VH-B by peptide linker 2, VH-B is linked to VL-A by peptide linker 3; and said peptide linker 1 and said peptide linker 3 are a peptide bond or have about 1 to about 10 amino acids; and
said peptide linker 2 has 3 to about 10 amino acids. - View Dependent Claims (2, 3, 4)
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Specification
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- Resources
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Current AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
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Original AssigneeDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
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InventorsLittle, Melvyn, Kipriyanov, Sergej
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Primary Examiner(s)Helms, Larry R.
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Assistant Examiner(s)Tungaturthi, Parithosh K.
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Application NumberUS09/674,794Time in Patent Office2,736 DaysField of Search536/23.4, 536/23.5, 536/23.53, 536/24.2, 536/24.4, 530/387.3, 530/388.22, 530/388.8, 530/389.7, 530/391.1, 530/391.7, 530/391.9, 424/135.1, 424/34.1, 424/192.1, 424/194.1, 435/320.1, 435/328US Class Current530/387.3CPC Class CodesC07K 14/82 Translation products from o...C07K 16/2803 against the immunoglobulin ...C07K 16/2809 against the T-cell receptor...C07K 2317/34 Identification of a linear ...C07K 2317/622 Single chain antibody (scFv)C07K 2319/00 Fusion polypeptideC07K 2319/02 containing a signal sequenceG01N 33/531 Production of immunochemica...
