Data frame selection for cytometer analysis
First Claim
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1. A method for detecting an optimum core stream in a channel, comprising:
- at a starting time, providing a sample of particles to form a core stream that flows in a channel;
directing light at the particles of the core stream flowing in the channel;
detecting light scattered by the particles in the core stream during a series of time intervals;
converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time;
analyzing the FALS versus SALS data for each interval of time; and
selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data.
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Abstract
A system for detecting when a core stream in a channel of a flow device is adequate for sufficient data accession. The system may determine the time period from the beginning of a sample run in a channel of a flow device to when the core stream is adequate. Data of detected FALS versus SALS light scattered by particles of the core stream may be plotted in data frames during a series of time intervals. The quality of the grouping and count rate of the data may indicate which frame and corresponding time interval reveal an adequate core stream.
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Citations
42 Claims
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1. A method for detecting an optimum core stream in a channel, comprising:
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at a starting time, providing a sample of particles to form a core stream that flows in a channel; directing light at the particles of the core stream flowing in the channel; detecting light scattered by the particles in the core stream during a series of time intervals; converting the light scattered by the particles detected at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle, into FALS versus SALS data for each interval of time of the series of time intervals after the starting time; analyzing the FALS versus SALS data for each interval of time; and selecting a time duration from the series of time intervals where the FALS versus SALS data exhibit an approximately certain count and grouping of data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. An optimization detection system comprising:
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a processor connected to a FALS and SALS detector mechanism situated proximate to a light source arrangement having a flow channel situated between the FALS and SALS detector mechanism and the light source arrangement; and wherein; the processor is for recording signals from the FALS and SALS detector mechanism; the processor is further for providing an output comprising a FALS signal versus a SALS signal for each particle detected in the flow channel; the output comprises a plurality of frames; each frame of the plurality of frames contains a plot of the FALS signal versus the SALS signal for each particle detected in the flow channel for a time interval; and the time interval is of a series of time intervals beginning at an entry of particles into the flow channel to form a core stream. - View Dependent Claims (16, 17, 18)
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19. A method for detecting an optimum core stream in a flow channel, comprising:
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beginning an insertion of particles to form a core stream sheathed by a fluid to flow in a channel; directing light at the particles in the channel; detecting and converting light scattered by the particles into electrical signals; processing the electrical signals in accordance with an algorithm for a determination of when the core stream is optimum for data taking; measuring the lapsed time between the insertion of particles to form the core stream and when the core steam is optimum for data taking; and plotting the electrical signals as FALS versus SALS data in a frame during a time interval; and wherein; the detecting light scattered by the particles is of light at a forward angle light scattering (FALS) angle and a small angle light scattering (SALS) angle; a series of time intervals occur during the lapsed time after the insertion of particles; and the frames of the time intervals are evaluated and an at least one frame is selected indicating that the core stream is optimum for data taking. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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30. Means for detecting an optimum core stream in a channel of a flow device, comprising:
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means for providing particles to form a core stream that flows in a channel starting at time zero; means for directing light at the particles of the core stream flowing in the channel; and means for determining an optimum core stream from light scattered by the particles; and wherein the means for determining an optimum core stream comprises; means for detecting light scattered by the particles at a set of scattering angles; means for converting detected light into data signals; means for analyzing the data signals for each time interval after time zero; and means for selecting a time interval where the data signals indicate an optimum core stream sufficient for data accession. - View Dependent Claims (31, 32, 33, 34, 35)
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36. A method for detecting an optimum core stream in a channel, comprising:
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providing a sample of particles to form a core stream that flows in a channel; directing light at the particles of the core stream flowing in the channel; detecting light scattered by the particles in the core stream during a series of time intervals; converting the light, scattered by the particles detected at a first light scattering angle and a second light scattering angle, into first light scattering angle versus second light scattering angle data for a series of time intervals; analyzing the first light scattering angle versus second light scattering angle data for the series of time intervals; and selecting a time duration from the series of time intervals where the first light scattering angle versus second light scattering angle data exhibit an approximately certain count and grouping of data. - View Dependent Claims (37, 38, 39, 40, 41, 42)
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Specification