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Method of determining a chemotherapeutic regimen based on ERCC1 expression

  • US 7,132,238 B2
  • Filed: 06/04/2003
  • Issued: 11/07/2006
  • Est. Priority Date: 12/01/2000
  • Status: Expired due to Term
First Claim
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1. A method of treating a tumor with a platinum-based chemotherapeutic regimen comprising:

  • (a) obtaining a tissue sample of the tumor and fixing the sample, to obtain a fixed tumor sample;

    (b) isolating mRNA from the fixed sample in the presence of an effective amount of a chaotrophic agent by first heating the tissue sample in a solution comprising an effective concentration of a chaotrophic compound to a temperature in the range of about 50 to about 100°

    C. for a time period of 5 to 120 minutes and recovering said mRNA from said chaotropic solution; and

    (c) subjecting the mRNA to amplification using a pair of oligonucleotide primers capable of amplifying a region of the Excision Repair Cross-Complementing 1 (ERCC1) gene, to obtain an amplified sample;

    (d) determining the amount of ERCC1 mRNA relative to the quantity of an internal control gene'"'"'s mRNA; and

    (f) treating the tumor, wherein the treatment comprises providing a platinum-based chemotherapeutic regimen comprising a genotoxic agent when the determined gene expression level for ERCC1 gene is below a predetermined threshold value.

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