Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
First Claim
1. A mutant of the soluble form of EC 1.1.5.2, also known as pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase (s-GDH), having a glycine residue present at a position corresponding to position 348 of the PQQ-dependent s-GDH wild-type sequence from A. calcoaceticus and having an amino acid sequence at least 90% identical thereto.
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Abstract
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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16 Claims
- 1. A mutant of the soluble form of EC 1.1.5.2, also known as pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase (s-GDH), having a glycine residue present at a position corresponding to position 348 of the PQQ-dependent s-GDH wild-type sequence from A. calcoaceticus and having an amino acid sequence at least 90% identical thereto.
- 12. A mutant PQQ-dependent s-GDH having glycine and proline residues present at positions corresponding to positions 348 and 428, respectively, of the PQQ-dependent s-GDH wild-type sequence from A. calcoaceticus and having an amino acid sequence at least 90% identical thereto.
Specification