Non-alloying core shell nanoparticles

• US 7,135,055 B2
• Filed: 03/26/2003
• Issued: 11/14/2006
• Est. Priority Date: 05/25/2001
• Status: Expired due to Term

• Current Assignee: Northwestern University
• Original Assignee: Nanosphere Incorporated (DiaSorin S.p.A.)
• Inventors: Mirkin, Chad A., Jin, Rongchao, Cao, Yun-Wei
• Primary Examiner(s): STRZELECKA, TERESA E
• Application Number:
  US10/397,579
• Publication Number:
  US 20040038255A1
• Time in Patent Office: 1,329 Days
• Field of Search: None
• US Class Current: 75/343
• CPC Class Codes: A61K 47/6923 the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
• CPC Class Codes: A61K 49/0063 characterised by a special physical or galenical form, e.g. emulsions, microspheres
• CPC Class Codes: B22F 1/054 Nanosized particles
• CPC Class Codes: B22F 1/10 Metallic powder containing lubricating or binding agents; Metallic powder containing organic material
• CPC Class Codes: B22F 1/102 Metallic powder coated with organic material
• CPC Class Codes: B22F 1/17 Metallic particles coated with metal
• CPC Class Codes: B22F 1/18 Non-metallic particles coated with metal
• CPC Class Codes: B22F 2998/00 Supplementary information concerning processes or compositions relating to powder metallurgy
• CPC Class Codes: B22F 9/24 starting from liquid metal compounds, e.g. solutions
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 25/00 Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C01B 19/007 Tellurides or selenides of metals C01B19/002 takes precedence
• CPC Class Codes: C01G 11/02 Sulfides
• CPC Class Codes: C01G 49/08 Ferroso-ferric oxide [Fe3O4]
• CPC Class Codes: C01G 7/00 Compounds of gold
• CPC Class Codes: C01P 2002/82 by IR- or Raman-data
• CPC Class Codes: C01P 2004/04 obtained by TEM, STEM, STM or AFM
• CPC Class Codes: C01P 2004/64 : Nanometer sized, i.e. from 1-100 nanometer
• CPC Class Codes: C01P 2004/84 : one phase coated with the other
• CPC Class Codes: C01P 2006/42 : Magnetic properties
• CPC Class Codes: C12Q 1/6813 : Hybridisation assays
• CPC Class Codes: C12Q 1/6816 : characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6834 : Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2563/155 : Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: G01N 33/543 : with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/5434 : using magnetic particle immunoreagent carriers which constitute new materials per se
• CPC Class Codes: G01N 33/54393 : Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/553 : Metal or metal coated
• CPC Class Codes: G01N 33/588 : with semiconductor nanocrystal label, e.g. quantum dots
• CPC Class Codes: H01F 1/0045 : Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use preparation of fullerenes in general C01B32/15
• CPC Class Codes: H01F 1/0054 : Coated nanoparticles, e.g. nanoparticles coated with organic surfactant
• CPC Class Codes: H01L 29/127 : Quantum box structures
• CPC Class Codes: Y10S 977/704 : Nucleic acids, e.g. DNA or RNA
• CPC Class Codes: Y10S 977/722 : On a metal substrate
• CPC Class Codes: Y10S 977/773 : Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/81 : Of specified metal or metal alloy composition
• CPC Class Codes: Y10T 428/12014 : having metal particles

Abstract

The present invention relates to composite core/shell nanoparticles and a two-step method for their preparation. The present invention further relates to biomolecule-core/shell nanoparticle conjugates and methods for their preparation. The invention also relates to methods of detection of biomolecules comprising the biomolecule or specific binding substance-core/shell nanoparticle conjugates.

Claims

1. A method for preparing core/shell nanoparticles comprising the steps of:

(a) providing inner metal-containing nanoparticle cores;

(b) treating the inner metal-containing nanoparticle cores simultaneously with a solution comprising a gold salt and a solution comprising a reducing agent at 0°

C. to produce a non-alloying gold shell surrounding the nanoparticle cores; and

(c) isolating the core/shell nanoparticles.

2. The method according to claim 1 wherein the gold salt comprises HAuCl₄, NaAuCl₄, KAuCl₄, or KAu(CN)₂.

3. The method according to claim 1 wherein the gold salt comprises HAuCl₄.

4. The method according to claim 1 wherein the reducing agent comprises NaBH₄ or ascorbic acid.

5. The method according to claim 4 wherein the reducing agent comprises NaBH₄.

6. The method of claim 1 wherein in step (b) the gold salt and reducing agent form a reaction mixture having a gold salt concentration of about 2 uM.

7. The method of claim 1 wherein the inner metal-containing nanoparticle cores comprise a metal oxide, Fe, Ni, Co, FePt or FeAu.