Compositions that specifically bind to colorectal cancer cells and methods of using the same

• US 7,135,333 B1
• Filed: 08/28/2000
• Issued: 11/14/2006
• Est. Priority Date: 08/07/1997
• Status: Expired due to Fees

• Current Assignee: Thomas Jefferson University (Main Line Health System)
• Original Assignee: Thomas Jefferson University (Main Line Health System)
• Inventors: Parkinson, Scott J., Barber, Michael T., Waldman, Scott A., Schulz, Stephanie, Pearlman, Joshua M.
• Primary Examiner(s): Rawlings, Stephen L.
• Application Number:
  US09/649,697
• Time in Patent Office: 2,269 Days
• Field of Search: 435/6, 435/7.23, 435/81, 435/320.1, 435/325, 536/23.5, 536/24.3, 536/24.31, 536/23.1, 536/24.33, 424/277.1, 514/44
• US Class Current: 435/325
• CPC Class Codes: A61K 2039/51 comprising whole cells, viruses or DNA/RNA
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: G01N 33/57419 of colon

Abstract

A unique transcription product, CRCA-1, and alternative translation products generated therefrom, are disclosed. The transcript and its translation products are markers for colorectal cells. Screening and diagnostic reagents, kits and methods for metastasized colorectal cancer are disclosed as are reagents, kits and methods for identifying adenocarcinomas as colorectal in origin. Compounds, compositions and methods of treating patients with metastasized colorectal cancer and for imaging metastasized colorectal tumors in vivo are disclosed. Compositions and methods for delivering active compounds such as gene therapeutics and antisense compounds to colorectal cells are disclosed. Vaccines compositions and methods of for treating and preventing metastasized colorectal cancer are disclosed.

Claims

1. An isolated nucleic acid molecule consisting of a fragment of the nucleic acid sequence of SEQ ID NO:

1 consisting of 30–

200 nucleotides including nucleotides 110–

113 of SEQ ID NO;

1.

2. The nucleic acid molecule of claim 1 consisting of 40–

100 nucleotides.

3. An isolated nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID NO:

1 or SEQ ID NO;

2.

4. An isolated recombinant vector comprising the nucleic acid molecule of claim 3.

5. An isolated host cell comprising the recombinant vector of claim 4.

6. The isolated nucleic acid of claim 3 consisting of the nucleic acid sequence of SEQ ID NO:

1.

7. An isolated oligonucleotide molecule that is the full complement of a fragment of SEQ ID NO:

1, said fragment consisting of 30–

200 nucleotides of SEQ ID NO;

1 including nucleotides 110–

113 of SEQ ID NO;

1.

8. The isolated oligonucleotide molecule of claim 7 consisting of 40–

100 nucleotides.

9. An isolated oligonucleotide molecule that is the full complement of a fragment of SEQ ID NO:

1, said fragment comprising 18 nucleotides of SEQ ID NO;

1 including nucleotides 110–

113 of SEQ ID NO;

1.

10. The oligonucleotide molecule of claim 9 consisting of 50 nucleotides.

11. An isolated oligonucleotide molecule comprising a nucleotide sequence that is the full complement of a fragment of SEQ ID NO:

1, said fragment comprising 18 nucleotides of SEQ ID NO;

1 including nucleotides 110–

113 of SEQ ID NO;

1;

wherein said oligonucleotide molecule hybridizes to a nucleic acid molecule consisting of SEQ ID NO;

1 without hybridizing to a nucleic acid molecule consisting of SEQ ID NO;

83.

12. The isolated oligonucleotide molecule of claim 11 comprising 50 nucleotides.

13. The isolated oligonucleotide molecule of claim 11 comprising 40 nucleotides.

14. The isolated oligonucleotide molecule of claim 11 comprising 25 nucleotides.

15. The isolated oligonucleotide molecule of claim 11 comprising 150 nucleotides.

16. The isolated oligonucleotide molecule of claim 11 comprising 28 nucleotides.

17. An isolated nucleic acid molecule that consists of SEQ ID NO:

2.

18. An isolated recombinant vector comprising the nucleic acid molecule of claim 17.

19. An isolated host cell comprising the recombinant vector of claim 18.