Apparatus and process for the lateral deflection and separation of flowing particles by a static array of optical tweezers

• US 7,137,574 B2
• Filed: 06/28/2004
• Issued: 11/21/2006
• Est. Priority Date: 09/13/2001
• Status: Expired due to Fees

• Current Assignee: University of Chicago
• Original Assignee: University of Chicago
• Inventors: Korda, Pamela T., Grier, David G.
• Primary Examiner(s): HWU, DAVIS D
• Application Number:
  US10/878,797
• Publication Number:
  US 20050001063A1
• Time in Patent Office: 876 Days
• Field of Search: 239/461, 239/463, 239/464, 239/466, 239/467, 239/483, 239/503, 239/513, 250/251, 359/566
• US Class Current: 239/461
• CPC Class Codes: B01D 2015/3895 using light
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 30/00 Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation G01N3/00, G01N5/00, G01N7/00, G01N9/00, G01N11/00, G01N13/00, G01N15/00, G01N17/00, G01N19/00, G01N21/00, G01N22/00, G01N23/00, G01N24/00, G01N25/00, G01N27/00, G01N29/00 take precedence
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G03H 2001/0077 for optical manipulation, e.g. holographic optical tweezers [HOT]

Abstract

A method and apparatus for laterally deflecting and/or separating a flow of particles using a static array of optical tweezers. In an array of optical tweezers with a lattice constant larger than the size of a particle of interest, particles driven past the array by an external force experience an additional interaction with the array of traps. By altering the angle of the array of traps relative to the external force, the particles'"'"' movement from trap to trap inside the array can be biased away from the direction of the external force, thereby enabling selective deflection and/or separation of particles.

Claims

1. An apparatus for controlled deflection of particles, comprising:

a plurality of particles;

an external bias force from a bias force source applied to the plurality of particles; and

an optical tweezer array having optical gradient forces associated therewith and the optical array oriented at a tilt angle relative to a direction of the bias force generated by the bias force source whereby the optical gradient forces of the optical tweezer array interact with the bias force to deflect the plurality of particles.

2. The apparatus as defined in claim 1 wherein the external bias force from the bias source and the optical tweezer array and the associated optical gradient forces are adjustable to a change in amount and direction of deflection of selected ones of the plurality of particles.

3. The apparatus as defined in claim 2 further including a diffractive optical element to generate the optical tweezer array, the diffractive optical element rotated by an angle to rotate the optical tweezer array with respect to flow direction of the plurality of particles, thereby enabling selected change of amount of deflection for the particles.

4. The apparatus as defined in claim 3 wherein the diffractive optical element is rotated to a particular range of angles which cause the plurality of particles to be deflected away from direction of flow of the particles.

5. The apparatus as defined in claim 3 wherein retrograde deflection with respect to the angle of rotation is achieved by means of generating a different local potential energy landscape of the optical tweezer array at selected angles of rotation for the diffractive optical element.

6. The apparatus as defined in claim 5 wherein the different local potential energy landscape causes formation of threshold angles of rotation of the optical tweezer array, thereby achieving locked in trajectories of the particles.

7. The apparatus as defined in claim 6 wherein a hierarchy is established to provide a form of Devil'"'"'s staircase of plateaus in transverse transport of the particles with increasing angle of rotation of the diffractive optical element.

8. The apparatus as defined in claim 2 wherein transverse deflection of the particles undergoes a change of sign with changing the angle of rotation of the optical tweezer array.

9. The apparatus as defined in claim 1 achieves the plurality of particles comprises different types of particles which respond differently to the optical tweezer array and the external bias force, thereby enabling sorting of the different type of particles.

10. The apparatus as defined in claim 1 wherein a continuous source of the plurality of particles is provided and continuous fractionated portions are produced.

11. The apparatus as defined in claim 1 further including means to monitor clogging of the apparatus and to remedy clogging by extinguishing the optical tweezer array.

12. The apparatus as defined in claim 1 further including a plurality of microfluidic channels disposed to collect the individual particle flow channels created by control of the optical array of the flow of particles created by the bias force from the bias force source.