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Electrochemical biosensor by screen printing and method of fabricating same

  • US 7,138,041 B2
  • Filed: 02/23/2004
  • Issued: 11/21/2006
  • Est. Priority Date: 02/23/2004
  • Status: Active Grant
First Claim
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1. An electrochemical biosensor, comprising:

  • an insulating substrate;

    a layer of electrically conductive wires disposed on said insulating substrate;

    an electrode layer comprising a plurality of electrodes including a reference electrode and at least one electrode, and at least two contact ports adapted to connect to a measuring device, said electrode layer being disposed on said layer of electrically conductive wires except for said reference electrode;

    a middle insulating layer disposed on said electrode layers without covering said electrodes and said contact ports, said middle insulating layer having a slot therein, above said electrodes;

    an active reaction layer having substances of reactant, reaction catalyst, mediator and surfactant spread on the surface of said electrodes and defining an electrode reaction area; and

    an upper cover adhered to said middle insulating layer,said upper cover having a closed chamber therein disposed at a rear end of said slot and extending above and in communication with said rear end of said slot, said slot defining a capillary inflow channel to enable a sample to be rapidly introduced into and fill said electrode reaction area by capillary action upon contact with a front tip of said capillary inflow channel and flow of said sample is stopped at a certain point along said capillary inflow channel due to said chamber.

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