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Mobile phase gradient generation microfluidic device

  • US 7,138,062 B2
  • Filed: 09/12/2005
  • Issued: 11/21/2006
  • Est. Priority Date: 02/26/2002
  • Status: Expired due to Term
First Claim
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1. A method for separating the components of a fluid sample, comprising:

  • (a) providing the microfluidic device comprisinga substrate having a microchannel formed in a surface thereof;

    a cover plate arranged over the substrate surface, the cover plate in combination with the microchannel defining a separation conduit for separating the components of the fluid sample according to a specific component property, wherein the separation conduit has an inlet port and an outlet port; and

    an integrated gradient-generation means for generating a gradient of a selected mobile-phase component in a mobile phase by the use of pressure driven flow, including a mechanical switching structure adapted to allow the mobile phase from the gradient-generation means to be transported through the inlet port into the separation conduit and out of the outlet port;

    (b) using the integrated gradient-generation means to generate a gradient of the selected mobile-phase component in the mobile phase,(c) controllably introducing a predetermined volume of the fluid sample from a sample source into the separation conduit; and

    (d) conveying the fluid sample through the separation conduit using a mobile phase, thereby separating the components of the fluid sample.

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