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Glucose-dependent insulin-secreting cells transfected with a nucleotide sequence encoding GLP-1

  • US 7,141,240 B2
  • Filed: 03/12/2002
  • Issued: 11/28/2006
  • Est. Priority Date: 03/12/2002
  • Status: Expired due to Fees
First Claim
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1. A method for constructing a glucose dependent insulin-secreting cell, in vitro, the method comprising the steps of:

  • providing an insulin-secreting cell, in vitro;

    isolating from a proglucagon gene a minigene construct consisting of (a) the coding region for a protein selected from the group consisting of glucagon-like peptide-1 (GLP-1) and an analog of GLP-1, said analog of GLP-1 being further selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 3;

    (b) a partial fragment of an IP sequence at the 5′

    -end; and

    (c) a nucleotide sequence at the 3′

    -end wherein the nucleotide sequence at the 3′

    -end is selected from the group consisting of, an entire IP-2 sequence and an entire IP-2 sequence along with a partial fragment of a glucagon-like peptide-2 (GLP-2) sequence;

    operably linking a promoter to the minigene construct;

    inserting or ligating the minigene construct into a plasmid; and

    transfecting the plasmid into the insulin-secreting cell,whereby said insulin-secreting cell produces GLP-1 or an analog of GLP-1 in a glucose dependent manner.

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