Treatment of substrates for immobilizing biomolecules

• US 7,141,374 B2
• Filed: 12/05/2003
• Issued: 11/28/2006
• Est. Priority Date: 08/09/2001
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Xie, Yuming, He, Mingqian, Li, Guangshan, Raghavachari, Nalini, Xie, Xinying, Bao, Yijia
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US10/729,618
• Publication Number:
  US 20040121391A1
• Time in Patent Office: 1,089 Days
• Field of Search: 435/6, 536/23.1, 436/24.3
• US Class Current: 435/6.11
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

Claims

1. A method of eliminating autofluorescence from a substrate coated with a silane comprising treating at least a portion of a first surface of the substrate with a reducing agent.

2. The method of claim 1, wherein the silane coating includes an amino-silane.

3. The method of claim 1, wherein the silane coating includes gamma-amino-propyl-silane.

4. The method of claim 1, wherein the reducing agent includes a hydride.

5. The method of claim 1, wherein the reducing agent includes sodium borohydride.

6. The method of claim 5, wherein the sodium borohydride is in a solution at a concentration ranging from 0.01% to 1% by volume.

7. The method of claim 5, wherein the sodium borohydride is in a solution at a concentration ranging from 0.2% to 0.3% by volume.

8. The method of claim 1, wherein the reducing agent includes sodium cyanoborohydride.

9. The method of claim 1, wherein the reducing agent includes copper sulfate.

10. The method of claim 1, wherein the reducing agent includes hydrogen.

11. The method of claim 1, wherein the substrate is an inorganic material.

12. The method of claim 11, wherein the inorganic material is a metal, a semiconductor material, a glass, or a ceramic material.

13. The method of claim 12, wherein the metal is gold, platinum, nickel, palladium, aluminum, chromium, steel, or gallium arsenide.

14. The method of claim 12, wherein the semiconductor material is silicon or germanium.

15. The method of claim 12, wherein the glass or ceramic material is quartz, glass, porcelain, alkaline earth aluminoborosilicate glass, or a mixed oxide.

16. The method of claim 1, wherein the substrate is an organic material.

17. The method of claim 16, wherein the organic material is a polyester, a polyvinylchloride, a polyvinylidene fluoride, a polytetrafluoroethylene, a polycarbonate, a polyamide, a poly(meth)acrylate, a polystyrene, or a polyethylene or ethylene/vinyl acetate copolymer.

18. The method of claim 1, wherein the substrate is a glass slide.

19. The method of claim 1, wherein prior to contacting the substrate with the reducing agent, covalently or non-covalently attaching an array of target biomolecule to the first surface of the substrate.