Multi-purpose optical analysis optical bio-disc for conducting assays and various reporting agents for use therewith

• US 7,141,416 B2
• Filed: 07/12/2002
• Issued: 11/28/2006
• Est. Priority Date: 07/12/2001
• Status: Expired due to Fees

• Current Assignee: Vindur Technologies, Inc.
• Original Assignee: Burstein Technologies, Inc.
• Inventors: Krutzik, Siegfried Richard
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): LUM, LEON YUN BON
• Application Number:
  US10/194,396
• Publication Number:
  US 20030096434A1
• Time in Patent Office: 1,600 Days
• Field of Search: 422/50, 422/55, 422 57- 58, 422/64, 422/68.1, 422/82.05, 422/82.09, 422/72, 435/4, 435/7.1, 435 74- 792, 435/287.1, 435/287.9, 435/288.7, 435/6, 435/287.2, 435/288.4, 435/288.5, 436/63, 436/164, 436/501, 436/45, 436/518, 436/524, 436/525, 436/43
• US Class Current: 435/288.5
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2800/52 Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

The present invention relates to optical bio-disc systems and related methods and to immobilizing receptor molecules. When a sample is injected into a fluidic circuit, the target agent binds to a capture probe bound in a capture zone. A signal is generated from tags attached to a reporter probe that has specific affinity to the target agent. The assays and methods of the present invention are implemented on an optical bio-disc. The optical bio-disc includes a flow channel having capture zones and in fluid communications with a mixing chamber, and a peripheral waste reservoir. The optical bio-disc is implemented on an optical bio-disc that has information encoding format such as CD. An disc drive assembly is employed to rotate the optical bio-disc, read and process any encoded information, and analyze the samples in the flow channel of the optical bio-disc.

Claims

1. An optical bio-disc, comprising:

a substantially circular substrate having a center and an outer edge;

a metal layer in operable connection with the substrate;

a target zone disposed between the center and the outer edge;

an active layer formed on the surface of said metal layer;

at least one capture agent that binds to said active layer such that the capture agent is immobilized on said active layer within the target zone to thereby form a capture zone, wherein the capture zone is located within the optical bio-disc;

a first circumferential reservoir located near the outer edge of said substrate and extending around the periphery of said substrate; and

a second circumferential reservoir located adjacent to and extending around an interior edge of the first circumferential reservoir, wherein the second circumferential reservoir is in fluid communication with the first circumferential reservoir through at least one port which extends through a common barrier between the first and second circumferential reservoirs, and wherein said common barrier physically and circumferentially supports a cap member placed on said substrate.

2. The optical bio-disc according to claim 1 wherein the metal layer is selected from the group comprising aluminum, gold, silver, nickel, and reflective metal alloys.

3. The optical bio-disc according to claim 1 wherein the substrate includes encoded information, the encoded information being readable by a disc drive assembly to control rotation of the bio-disc.

4. The optical bio-disc according to claim 1 further comprising an enzyme, wherein the enzyme, when exposed to an enzyme substrate, produces a signal detectable by an incident beam of electromagnetic radiation.

5. The optical bio-disc according to claim 1 further comprising a flow channel in fluid communication with said capture zone and said first and second circumferential reservoirs, and an input site in fluid communication with said flow channel.

6. The optical bio-disc according to claim 1, further comprising a plurality of fluidic channels, each of said fluidic channels being in fluid communication with the first and second circumferential reservoirs.

7. The optical bio-disc according to claim 1 further comprising at least one absorber pad in fluid communication with the first circumferential reservoir.

8. The optical bio-disc according to claim 7, wherein the at least one absorber pad comprises a plurality of absorber pads positioned within the first circumferential reservoir at spaced locations so as to promote and maintain balance of the optical bio-disc when said bio-disc is in rotation.

9. The optical bio-disc according to claim 1, further comprising a drying agent or desiccant configured to absorb moisture so as to preserve functional activity of reagents deposited in the optical bio-disc.

10. The optical bio-disc according to claim 9, wherein the drying agent or desiccant is contained in an absorber pad located in fluid communication with the first circumferential reservoir.

11. An optical bio-disc, comprising:

a substantially circular substrate having a center and an outer edge;

a target zone disposed between the center and the outer edge;

at least one capture probe located within the target zone, wherein the capture probe is configured to immobilize a target agent from a sample within the target zone;

a first circumferential reservoir located near the outer edge of said substrate and extending around the periphery of said substrate; and

a second circumferential reservoir located adjacent to and extending around an interior edge of the first circumferential reservoir, wherein the second circumferential reservoir is in fluid communication with the first circumferential reservoir through at least one port which extends through a common barrier between the first and second circumferential reservoirs, and wherein said common barrier physically and circumferentially supports a cap member placed on said substrate.

12. The optical bio-disc according to claim 11, further comprising at least one absorber pad located in fluid communication with the first circumferential reservoir.

13. The optical bio-disc according to claim 12, wherein the absorber pad includes a drying agent or desiccant.

14. The optical bio-disc according to claim 12, wherein the at least one absorber pad comprises a plurality of absorber pads positioned within the first circumferential reservoir at spaced locations so as to promote and maintain balance of the optical bio-disc when said bio-disc is in rotation.

15. An optical bio-disc, comprising:

a substantially circular substrate having a center and an outer edge;

a metal layer in operable connection with the substrate;

a target zone disposed between the center and the outer edge of said substrate;

an active layer formed on the surface of said metal layer;

at least one capture agent that binds to said active layer such that the capture agent is immobilized on said active layer within the target zone to thereby form a capture zone, wherein the capture zone is located within the optical bio-disc, and wherein the active layer is formulated to immobilize a pellet formed by an enzyme reaction;

a first circumferential reservoir located near the outer edge of said substrate and extending around the periphery of said substrate;

a second circumferential reservoir located adjacent to and extending around an interior edge of the first circumferential reservoir, wherein the second circumferential reservoir is in fluid communication with the first circumferential reservoir through at least one port which extends through a common barrier between the first and second circumferential reservoirs wherein said common barrier physically and circumferentially supports a cap member placed on said substrate; and

a drying agent or desiccant in fluid communication with the first circumferential reservoir and configured to absorb moisture so as to preserve functional activity of reagents deposited in the optical bio-disc.

16. An optical bio-disc, comprising:

a substantially circular substrate having a center and an outer edge;

a metal layer in operable connection with the substrate;

a target zone disposed between the center and the outer edge;

an active layer formed on the surface of said metal layer;

a plurality of fluidic channels in fluid communication with the active layer;

at least one capture agent located in at least one of the fluidic channels, wherein said capture agent binds to said active layer such that the capture agent is immobilized on said active layer within the target zone to thereby form a capture zone, wherein the capture zone is located within the optical bio-disc;

a first circumferential reservoir located near the outer edge of said substrate and extending around the periphery of said substrate; and

a second circumferential reservoir located adjacent to and extending around an interior edge of the first circumferential reservoir, wherein the second circumferential reservoir is in fluid communication with the first circumferential reservoir through at least one port which extends through a common barrier between the first and second circumferential reservoirs, wherein said common barrier physically and circumferentially supports a cap member placed on said substrate, and wherein the second circumferential reservoir is in fluidic communication with said plurality of fluidic channels.

17. The optical bio-disc according to claim 16 further comprising at least one absorber pad in fluid communication with the first circumferential reservoir.

18. The optical bio-disc according to claim 17, wherein the at least one absorber pad comprises a plurality of absorber pads positioned within the first circumferential reservoir at spaced locations so as to promote and maintain balance of the optical bio-disc when said bio-disc is in rotation.