Glyphosate resistant plants using hybrid promoter constructs

• US 7,141,722 B2
• Filed: 08/18/2004
• Issued: 11/28/2006
• Est. Priority Date: 12/16/1999
• Status: Expired due to Term

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Flasinski, Stanislaw, Fincher, Karen L, Wilkinson, Jack Q
• Primary Examiner(s): Nguyen, Dave Trong
• Assistant Examiner(s): Marvich, Maria
• Application Number:
  US10/920,869
• Publication Number:
  US 20050022261A1
• Time in Patent Office: 832 Days
• Field of Search: None
• US Class Current: 800/300
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells
• CPC Class Codes: C12N 15/8221 Transit peptides
• CPC Class Codes: C12N 15/8222 Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
• CPC Class Codes: C12N 15/8223 Vegetative tissue-specific promoters
• CPC Class Codes: C12N 15/823 Reproductive tissue-specific promoters
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8286 for insect resistance
• CPC Class Codes: C12N 2710/00043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12N 9/1092 3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.

Claims

1. Transgenic plant seed comprising one or more DNA expression constructs, said constructs comprising a hybrid promoter DNA sequence comprising the 34S FMV promoter operably linked to an Arabidopsis EF1α

promoter;

a structural DNA sequence encoding a glyphosate tolerance protein; and

a 3′

non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′

end of the RNA sequence;

wherein the structural DNA sequence is operably linked to the hybrid promoter and the 3′

non-translated region.

2. The transgenic plant seed of claim 1, wherein the gylphosate tolerance protein is a glyphosate oxidoreductase (GOX) protein.

3. The transgenic plant seed of claim 1, wherein the glyphosate tolerance protein is an 5-enolpyruvyl-3-phosphoshikimate synthase (EPSPS) protein.

4. The transgenic plant seed of claim 3, wherein the glyphosate tolerance protein is aroA:

CP4 EPSPS.

5. The transgenic plant seed of claim 1, wherein the DNA sequence encoding the glyphosate tolerance protein is further operably linked to a structural DNA sequence encoding a chloroplast transit peptide.

6. The transgenic plant seed of claim 1, wherein the FMV promoter comprises a duplicated enhancer (eFMV).

7. The transgenic plant seed of claim 1, wherein the hybrid promoter is SEQ ID NO:

28.

8. A transgenic plant grown from the seed of any one of claims 1–

5, and 6–

7.

9. The transgenic plant of claim 8, further characterized as having vegetative and reproductive glyphosate tolerance.

10. The transgenic plant of claim 9, wherein the plant is capable of tolerating exposure to glyphosate at a rate up to 256 ounces/acre.

11. The transgenic plant of claim 9, wherein the plant is capable of tolerating exposure to glyphosate at a rate ranging from 16 to 64 ounces/acre.

12. The transgenic plant of claim 8, wherein said plant is a monocot crop species.

13. The transgenic plant of claim 8, wherein said plant is a dicot crop species.

14. The transgenic plant of claim 12, wherein said monocot crop is barley, corn, rice, rye, sorghum or wheat.

15. The transgenic plant of claim 13, wherein said dicot crop is alfalfa, tomato, soybean, cotton, canola or sunflower.