×

Methods for selectively stimulating proliferation of T cells

  • US 7,144,575 B2
  • Filed: 03/17/2003
  • Issued: 12/05/2006
  • Est. Priority Date: 11/23/1988
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for expanding a population of CD8+ T cells in number of from about 100 to about 100,000 fold over the original CD8+ T cell population or to about 10 log10 to 12 log10, comprising;

  • a) contacting a population of CD8+ T cells with a surface comprising1) an anti-CD3 antibody or a CD-3-binding fragment thereof; and

    2) a second agent which stimulates a CD28 antigen, wherein the second agent is selected from the group consisting of an anti-CD28 antibody or a CD28-binding fragment thereof, B7-1 or a CD28-binding fragment thereof, B7-2 or a CD28-binding fragment thereof, a B7-1Ig fusion protein that interacts with CD28 and a B7-2Ig fusion protein that interacts with CD28, wherein said anti-CD3 antibody or CD3-binding fragment thereof, and said second agent are attached on the same surface;

    b) adding exogenous IL-2 to the population of CD8+ T cells, such that the population of CD8+ T cells increase in number from about 100 to about 100,000 fold over the original CD8+ T cell population or to about 10 log10 to 12 log10.

View all claims
  • 5 Assignments
Timeline View
Assignment View
    ×
    ×