Diagnostic devices containing porous material

• US 7,148,056 B2
• Filed: 03/24/2001
• Issued: 12/12/2006
• Est. Priority Date: 03/24/2000
• Status: Expired due to Fees

• Current Assignee: Mark B. Lyles
• Original Assignee: Mark B. Lyles
• Inventors: Lyles, Mark B.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/817,016
• Publication Number:
  US 20020064781A1
• Time in Patent Office: 2,089 Days
• Field of Search: 424/400, 435/287.1, 435/287.2, 435/287.9, 435/288.3, 436518-532
• US Class Current: 435/287.2
• CPC Class Codes: B29C 41/16 Slip casting, i.e. applying a slip or slurry on a perforated or porous or absorbent surface with the liquid being drained away
• CPC Class Codes: C04B 14/46 Rock wool ; Ceramic or silicate fibres C04B14/40, C04B14/42 take precedence
• CPC Class Codes: C04B 2111/00836 for medical or dental applications
• CPC Class Codes: C04B 22/0013 Boron compounds
• CPC Class Codes: C04B 30/02 containing fibrous materials
• CPC Class Codes: C04B 38/06 by burning-out added substances by burning natural expanding materials or by sublimating or melting out added substances
• CPC Class Codes: C04B 40/0028 Aspects relating to the mixing step of the mortar preparation
• CPC Class Codes: C04B 40/006 involving the elimination of excess water from the mixture
• CPC Class Codes: C04B 40/0268 Heating up to sintering temperatures C04B41/0072 takes precedence
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

Diagnostic devices are disclosed which contain porous material. The porous material preferably comprises silica and alumina. Chemical and/or biological molecules can be bound to the porous material in high concentrations while both having high accessibility to molecules in solution, and retaining their natural conformation. Such diagnostic devices may be used for a wide array of assays including protein (e.g. ELISA) and nucleic acid (e.g. hybridization on chips or beads) detection and/or quantification methods.

Claims

1. A diagnostic device comprising:

a rigid three-dimensional porous material manufactured from alumina, silica and a fusion source, said material having mean pore diameters greater than about 10 microns and a density equal to or greater than 6 pounds per cubic foot, said material is in a form selected from the group of forms consisting of plates, cylinders, discs, particles, tubes, beads, coatings, and any combination thereof, wherein compounds selected from the group consisting of antibodies, oligonucleotides, DNA, RNA, peptides, proteins, oligosaccharides, enzymes, receptors, and any combination thereof are bound to the material.

2. The device of claim 1 wherein the material is manufactured from a boron fusion source.

3. The device of claim 1 wherein the material is manufactured from a boron nitride fusion source.

4. The device of claim 1 wherein the material is silanated.

5. The device of claim 1 wherein an exposed surface of the material comprises at least 50% silicon dioxide.

6. The device of claim 1 wherein an exposed surface of the material has been modified by a chemical reaction.

7. The device of claim 1 wherein the material is manufactured from about 1% to about 50% by weight alumina.

8. The device of claim 7 wherein the material is further manufactured from about 50% to about 98% by weight silica.

9. The device of claim 8 wherein the material is further manufactured from about 1% to about 5% by weight of a fusion source comprising boron.