Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acid levels

• US 7,148,336 B2
• Filed: 10/11/2002
• Issued: 12/12/2006
• Est. Priority Date: 08/26/1999
• Status: Expired due to Term

• Current Assignee: Monsanto Company (Bayer AG)
• Original Assignee: Calgene Incorporated (Pharmacia Corp.)
• Inventors: Fillatti, Joanne
• Primary Examiner(s): McElwain, Elizabeth F.
• Application Number:
  US10/268,754
• Publication Number:
  US 20030084480A1
• Time in Patent Office: 1,523 Days
• Field of Search: 536/23.1
• US Class Current: 536/23.1
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.

Claims

1. An isolated polynucleotide comprising SEQ ID NO:

24.

2. An isolated polynucleotide comprising at least 50 consecutive nucleotides of SEQ ID NO:

24.

3. An isolated polynucleotide comprising at least 30 consecutive nucleotides of SEQ ID NO:

24.

4. An isolated polynucleotide having at least 90% identity to SEQ ID NO:

24.

5. The isolated polynucleotide of claim 4 having at least 95% identity to SEQ ID NO:

24.

6. The isolated polynucleotide of claim 4 having at least 97% identity to SEQ ID NO:

24.

7. The isolated polynucleotide of claim 4 having at least 98% identity to SEQ ID NO:

24.

8. The isolated polynucleotide of claim 4 having at least 99% identity to SEQ ID NO:

24.