Method and apparatus for detecting biomolecular interactions

• US 7,148,964 B2
• Filed: 04/13/2006
• Issued: 12/12/2006
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Li, Peter Y., Cunningham, Brian T., DiFilippi, Frank
• Primary Examiner(s): Lee, Hwa (Andrew)
• Assistant Examiner(s): GEISEL, KARA E
• Application Number:
  US11/404,162
• Publication Number:
  US 20060181704A1
• Time in Patent Office: 243 Days
• Field of Search: 356/326
• US Class Current: 356/326
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/0635 Structured illumination, e.g. with grating
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 2201/0833 Fibre array at detector, resolving
• CPC Class Codes: G01N 2201/0846 Fibre interface with sample, e.g. for spatial resolution
• CPC Class Codes: G01N 2201/1248 Validating from signal shape, slope, peak
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: G02B 5/1814 structurally combined with one or more further optical elements, e.g. lenses, mirrors, prisms or other diffraction gratings G02B5/189 takes precedence

Abstract

Method and apparatus for detecting biomolecular interactions. The use of labels is not required and the methods may be performed in a high-throughput manner. An apparatus for detecting biochemical interactions occurring on the surface of a biosensor includes a light source. A first optical fiber is coupled to the light source and illuminates the biosensor. A second optical fiber detects a wavelength reflected from the biosensor. A spectrometer determines spectra of a reflected signal from the biosensor.

Claims

1. A biosensor imaging system comprising:

a light source generating white light;

a collimator for collimating said white light, said collimated light being directed onto a surface of a biosensor;

an imaging spectrometer receiving reflected light from said biosensor, and

software calculating a peak wavelength value (PWV) of said reflected light.

2. The invention of claim 1 wherein said light source is a white light source.

3. The invention of claim 1 wherein said biosensor is arranged as an array of rows and columns of discrete detection areas.

4. The invention of claim 3 wherein said biosensor is coupled to a liquid containing vessel.

5. The invention of claim 1 wherein said imaging system further comprises an illuminating optical fiber coupled to the light source and wherein said illuminating optical fiber illuminates a bottom surface of said biosensor.

6. The invention of claim 1 further comprising an optical fiber for illuminating said biosensor.

7. The invention of claim 1 wherein said software enables a visual representation of said calculated PWV of said reflected light.