RNA detection assays

• US 7,150,982 B2
• Filed: 02/26/2002
• Issued: 12/19/2006
• Est. Priority Date: 09/09/1991
• Status: Expired due to Fees

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Third Wave Technologies Incorporated (Hologic Incorporated)
• Inventors: Ma, WuPo, Ip, Hon S., Ji, Lin, Chehak, LuAnne, Argue, Brad T., Olson, Sarah M., Thompson, Lisa C., Bartholomay, Christian Tor, Olson-Munoz, Marilyn C., Lyamicheva, Natalie E., Neri, Bruce P., Eis, Peggy S., Hall, Jeff G., Skrzypczynski, Zbigniev, Vedvik, Kevin L., Lukowiak, Andrew A., Schaefer, James J., Lyamichev, Victor, Curtis, Michelle L., Takova, Tsetska Y., Allawi, Hatim T., Kwiatkowski, Robert W., Kaiser, Michael W.
• Primary Examiner(s): Patterson, Jr., Charles L.
• Application Number:
  US10/084,839
• Publication Number:
  US 20030186238A1
• Time in Patent Office: 1,757 Days
• Field of Search: 435/199, 435/6
• US Class Current: 435/199
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/6823 Release of bound markers
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: C12Q 2537/162 Helper probe
• CPC Class Codes: C12Q 2561/109 Invader technology
• CPC Class Codes: H04L 65/1101 Session protocols
• CPC Class Codes: H04L 69/32 Architecture of open systems interconnection [OSI] 7-layer type protocol stacks, e.g. the interfaces between the data link level and the physical level
• CPC Class Codes: H04L 9/40 Network security protocols

Abstract

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Claims

1. A kit comprising a recombinant enzyme comprising a 5′

nuclease, wherein said recombinant enzyme comprises a polypeptide encoded by the nucleic acid shown in SEQ ID NO;

2856.

2. The kit of claim 1, wherein said polypeptide is shown in SEQ ID NO:

2857.

3. The kit of claim 1, further comprising a probe oligonucleotide.

4. The kit of claim 2, further comprising an INVADER oligonucleotide.

5. The kit of claim 1, further comprising a labeled oligonucleotide.