RNA detection assays
First Claim
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1. A kit comprising a recombinant enzyme comprising a 5′
- nuclease, wherein said recombinant enzyme comprises a polypeptide encoded by the nucleic acid shown in SEQ ID NO;
2856.
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Abstract
The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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5 Claims
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1. A kit comprising a recombinant enzyme comprising a 5′
- nuclease, wherein said recombinant enzyme comprises a polypeptide encoded by the nucleic acid shown in SEQ ID NO;
2856. - View Dependent Claims (2, 3, 4, 5)
- nuclease, wherein said recombinant enzyme comprises a polypeptide encoded by the nucleic acid shown in SEQ ID NO;
Specification