Process for filling microfluidic channels

• US 7,150,999 B1
• Filed: 03/05/2002
• Issued: 12/19/2006
• Est. Priority Date: 03/09/2001
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Shuck, Gary L.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Nagpaul, Jyoti
• Application Number:
  US10/092,011
• Time in Patent Office: 1,750 Days
• Field of Search: 422 99-101, 435/254.1, 436/180, 438/126
• US Class Current: 436/180
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention provides novel methods and devices for filling at least one microfluidic channel with a gas or with a degassed fluid. In particular the methods and devices of the invention are useful in completely filling substantially all areas of a microfluidic device with a gas or with a degassed fluid without incorporating bubbles within such microfluidic devices.

Claims

1. A method of filling at least one microfluidic element of a microfluidic device with a gas or fluid, the method comprising:

placing the microfluidic device in a vacuum chamber;

applying a vacuum to the vacuum chamber;

while the microfluidic device remains under vacuum, introducing the gas or fluid into the vacuum chamber such that the microfluidic device is submerged in to gas or fluid;

venting the at least one microfluidic element to the gas or fluid; and

filling the at least one microfluidic element with the gas or fluid.

2. The method of claim 1 wherein applying a vacuum comprises applying a vacuum between about 0.5 and 102 kPa.

3. The method of claim 1 wherein applying a vacuum comprises applying a vacuum between about 15 and 85 kPa.

4. The method of claim 1 wherein applying a vacuum comprises applying a vacuum between about 30 and 70 kPa.

5. The method of claim 1 wherein applying a vacuum comprises applying a vacuum between about 45 and 55 kPa.

6. The method of claim 1 wherein applying a vacuum comprises applying a vacuum between about 0.5 and 5 kPa.

7. The method of claim 1 wherein filling the at least one microfluidic element with the gas or fluid comprises filling the at least one microfluidic channel with a degassed fluid.

8. The method of claim 1 wherein filling the at least one microfluidic element with the gas or fluid comprises filling the at least one microfluidic element with at least one fluid selected from a group consisting of water, buffer, EDTA solution, DMSO, PEG, polyacrylamide, and NaOH solution.

9. The method of claim 1 wherein filling the at least one microfluidic element with the gas or fluid comprises diffusing the gas or fluid into the at least one microfluidic element.

10. The method of claim 1 wherein the at least one microfluidic element is fluidly connected to an at least one capillary element, which element includes a capillary channel disposed therein.

11. The method of claim 1 wherein the at least one microfluidic element comprises a plurality of microfluidic channels.

12. The method of claim 11 wherein the plurality of microfluidic channels are fluidly coupled to one or more micro-reservoirs.

13. The method of claim 1 wherein filling the at least one microfluidic element with the gas or fluid comprises filling the at least one microfluidic element with at least one inert gas.

14. The method of claim 13 wherein the at least one inert gas is selected from a group consisting of carbon dioxide and nitrogen.

15. The method of claim 1 wherein filling the at least one microfluidic element with the gas or fluid comprises filling the at least one microfluidic element with both a gas and a fluid.

16. A system for filling a microfluidic element of a microfluidic device with a gas or a fluid, the system comprising:

a) a chamber configured to receive the microfluidic device;

b) a vacuum source which is fluidly coupled to the chamber and which is configured to apply a vacuum to the chamber;

c) at least one source of a gas or fluid which is fluidly coupled to the chamber and which is configured to introduce at least one of a gas or a fluid into the chamber; and

d) a detector configured to monitor filling of the microfluidic element of the microfluidic device with the gas or fluid.

17. The system of claim 16 wherein the microfluidic element comprises at least one microfluidic channel.

18. The system of claim 16 wherein the vacuum source is applicable to apply a vacuum between about 0.5 and 102 kPa to the chamber.

19. The system of claim 16 wherein the vacuum source is applicable to apply a vacuum between about 15 and 85 kPa to the chamber.

20. The system of claim 16 wherein the vacuum source is applicable to apply a vacuum between about 30 and 70 kPa to the chamber.

21. The system of claim 16 wherein the vacuum source is applicable to apply a vacuum between about 0.5 and 5 kPa to the chamber.

22. The system of claim 16 further comprising a processor operably coupled to the microfluidic device, wherein the processor comprises an instruction set for acquiring data from the detector and for controlling filling of the microfluidic device with the gas or the fluid.