Devices and methods for programmable microscale manipulation of fluids

• US 7,152,616 B2
• Filed: 12/04/2003
• Issued: 12/26/2006
• Est. Priority Date: 12/04/2002
• Status: Expired due to Fees

• Current Assignee: Noble Venture Finance II SA
• Original Assignee: Spinx Company Incorporated
• Inventors: Van de Vyver, Bart, Zucchelli, Piero
• Primary Examiner(s): Krishnamurthy, Ramesh
• Application Number:
  US10/491,299
• Publication Number:
  US 20050109396A1
• Time in Patent Office: 1,118 Days
• Field of Search: 137/67, 137/68.11, 137/72, 137/74, 422/64, 422/103, 436/45, 436/180
• US Class Current: 137/68.11
• CPC Class Codes: B01F 31/10 with a mixing receptacle rotating alternately in opposite directions
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/451 wherein the mixture is directly exposed to an electromagnetic field without use of a stirrer, e.g. for material comprising ferromagnetic particles or for molten metal
• CPC Class Codes: B01F 35/713 comprising breaking packages or parts thereof, e.g. piercing or opening sealing elements between compartments or cartridges containers or packages per se B65D
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: B81C 1/00119 Arrangement of basic structures like cavities or channels, e.g. suitable for microfluidic systems
• CPC Class Codes: B81C 2201/019 Bonding or gluing multiple substrate layers
• CPC Class Codes: F16K 2099/0073 Fabrication methods specifically adapted for microvalves
• CPC Class Codes: F16K 2099/008 : Multi-layer fabrications
• CPC Class Codes: F16K 2099/0084 : Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0001 : Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/003 : Valves for single use only
• CPC Class Codes: F16K 99/0034 : Operating means specially adapted for microvalves
• CPC Class Codes: F16K 99/004 : using radiation
• CPC Class Codes: G01N 2001/2886 : Laser cutting, e.g. tissue catapult
• CPC Class Codes: G01N 2035/00247 : Microvalves
• CPC Class Codes: G01N 21/01 : Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 35/00069 : whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• CPC Class Codes: Y10T 137/1624 : Destructible or deformable element controlled
• CPC Class Codes: Y10T 137/1632 : Destructible element
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

The present invention is directed generally to devices and methods for controlling fluid flow in meso-scale fluidic components in a programmable manner. Specifically, the present invention is directed to an apparatus and method for placing two microfluidic components in fluid communication at an arbitrary position and time, both of which are externally defined. The inventive apparatus uses electromagnetic radiation to perforate a material layer having selected adsorptive properties. The perforation of the material layer allows the fluid communication between microfluidic components. Other aspects of this invention include an apparatus and method to perform volumetric quantitation of fluids, an apparatus to program arbitrary connections between a set of input capillaries and a set of output capillaries, and a method to transport fluid in centripetal device from a larger to a smaller radius. In addition, the present invention also is directed to a method to determine the radial and polar position of a pickup in the reference frame of a rotating device.

Claims

1. An apparatus for processing fluids comprising:

a first substrate comprising a plurality of first fluidic components;

a second substrate comprising a plurality of second fluidic components corresponding to the first fluidic components;

a material layer separating the plurality of first fluidic components from the plurality of second fluidic components said material layer is formed of a polymeric material loaded with a dye; and

electromagnetic radiation generating means for generating a selected electromagnetic radiation for directing onto the layer of material at a position corresponding to a portion of the layer located between at least a pair of corresponding fluidic components from the plurality of first fluidic components and the plurality of second fluidic components said selected electromagnetic radiation causing perforation of the material layer at the position allowing fluid communication between at least a pair of fluidic components.

3. The apparatus according to claim 1, wherein said selected electromagnetic radiation is selected from the group consisting of infrared, visible and ultra-violet spectrum.

4. The apparatus according to claim 1, wherein said electromagnetic radiation generating means is selected from the group consisting of a laser, compact disk drive pickup and digital versatile disk drive pickup.

5. The apparatus according to claim 1, wherein said material layer includes a thickness from about 0.5 μ

M to about 100 μ

M.

6. The apparatus according to claim 1, wherein said material layer is selected from the group consisting of polymer foils and metallic foils.

7. The apparatus according to claim 1, wherein said material layer is a foil formed from a material selected from the group consisting of polymers, copolymers, monomers, metals, waxes, polysaccharides and liquid crystal polymers.

8. The apparatus according to claim 1, wherein said dye has optical properties that are substantially matched to said selected electromagnetic radiation.

9. The apparatus according to claim 1, wherein said material layer is treated to substantially absorb said selected electromagnetic radiation, said treatment is selected from the group consisting of dye loading, chemical surface treatment, chemical loading, optical interference and optical polarization.

10. The apparatus according to claim 1, wherein said material layer is formed of multiple layers having selected absorption properties and, wherein said absorption properties are responsive to said selected radiation.

11. The apparatus according to claim 1, wherein said material layer is formed of a polymeric material selected from the group consisting of Poly(methyl methacrylate) (PMMA), Low Density Polyethylene (LDPE), Linear Low Density Polyethylene (LLDPE), High Density Polyethylene (HDPE), Polyethylene Teraphathalate (PET), Polyethylene (PE), p olycarbonate (PC), Polyethylene Terephthalate Glycol (PETG) Polystyrene (PS), Ethyl Vinyl Acetate (EVA), and polyethylene napthalate (PEN).

12. The apparatus according to claim 1, wherein said first and second substrate are in the form of a disk wherein rotation of said apparatus produces a centripetal force on a fluid contained in one or more of said first and second fluidic components, said centripetal force causing fluids to move to an outer radial position.

13. The apparatus according to claim 1, wherein the substrate materials are selected from the group consisting of polymers, monomers, co-polymers, resins, ceramic, glass, quartz and silicon.

14. The apparatus according to claim 1, wherein said first and second substrate further contain optical components selected from the group consisting of lenses, mirrors and prisms.

15. The apparatus according to claim 1, further containing at least one more additional substrate and at least one more material layer.

16. The apparatus according to claim 1, wherein said selected radiation has a desired intensity from about 1 4J to about 100 4J and exposure time from about 1 microsecond and about 100 μ

M.

17. The apparatus according to claim 16, wherein said desired intensity and exposure time is about 10 μ

J and about 10 μ

s, respectively.

18. The apparatus according to claim 16, wherein said selected radiation has a pulse geometry.

19. The apparatus according to claim 18, wherein said pulse geometry and said desired exposure time does not substantially alter a sample of interest.

20. The apparatus according to claim 1, wherein at least one substrate has an optical window allowing for on board sample detection.

21. The apparatus according to claim 1, wherein at least one substrate has a removable portion allowing for off board sample detection.

22. The apparatus according to claim 21, wherein said removable portion is a MALDI foil.

23. The apparatus according to claim 1, wherein a portion of said selected electromagnetic radiation directed upon said material layer is reflected or transmitted into a means for allowing confirmation of perforation.

2. An apparatus for processing fluids comprising:

a first substrate comprising a plurality of first fluidic components;

a second substrate comprising a plurality of second fluidic components corresponding to the first fluidic components;

a material layer separating the plurality of first fluidic components from the plurality of second fluidic components;

an electromagnetic generating means for generating a selected electromagnetic radiation for directing onto the layer of material at a position corresponding to a portion of the layer located between at least a pair of corresponding fluidic components from the plurality of first fluidic components and the plurality of second fluidic components said selected electromagnetic radiation causing perforation of the material layer at the position allowing fluid communication between at least a pair of fluidic components; and

a feedback generating means for generating an optical feedback to said electromagnetic generating means whereby perforation of said material layer is confirmed.

24. An apparatus for multiplexing fluids comprising:

a first substrate comprising a set of input capillaries;

a second substrate comprising a set of output capillaries corresponding to the set of input capillaries;

a material layer positioned between said first substrate and said second substrate forming a valving interface between each of said input capillaries, and said output capillaries corresponding thereto said material layer is formed of a polymeric material loaded with a dye; and

a means for generating electromagnetic radiation, said generating means producing a selected radiation for directing onto said material layer said selected radiation causing perforation at a said valving interface causing fluid communication between said input capillary and said output capillary.

25. The apparatus according to claim 24, further comprising a means for optical feedback wherein said generating means produces a selected radiation for directing onto said material layer and said optical feedback means signals said generating means when said perforation occurs.

26. A method for processing fluids comprising:

providing a first substrate comprising a plurality of first fluidic components;

providing a second substrate comprising a plurality of second fluidic components corresponding to the first fluidic components;

providing a material layer separating the plurality of first fluidic components from the plurality of second fluidic components said material layer contains an optical dye having absorptive properties that absorb said electromagnetic radiation causing perforation; and

directing electromagnetic radiation onto said material layer in at least one position corresponding to at least one selected position between at least a pair of corresponding fluidic components from the plurality of first fluidic components and the plurality of second fluidic components, said electromagnetic radiation causing perforation in at least one selected position thereby allowing fluid communication between at least one pair of fluidic components.

27. The method according to claim 26, wherein said electromagnetic radiation is selected from the group consisting of infrared, visible and ultra-violet spectrum.

28. The method according to claim 26, wherein said material layer includes a thickness from about 0.5 μ

M to about 100 μ

M.

29. The method according to claim 26, wherein said material layer is selected from the group consisting of polymer foils and metallic foils.

30. The method according to claim 26, wherein said material layer is a foil formed from a material selected from the group consisting of polymers, copolymers, monomers, metals, waxes, polysaccharides and liquid crystal polymers.

31. The method according to claim 26, wherein said material layer is formed of a polymeric material loaded with a dye.

32. The method according to claim 31, wherein said dye has optical properties and said optical properties are substantially matched to said selected radiation.

33. The method according to claim 26, wherein said material layer is treated to substantially absorb said selected radiation, said treatment is selected from the group consisting of dye loading, chemical surface treatment, chemical loading, optical interference and optical polarization.

34. The method according to claim 26, wherein said material layer is formed of multiple layers having selected absorption properties wherein said absorption properties are responsive to said selected radiation.

35. The method according to claim 26, wherein said material layer is formed of a polymeric material selected from the group consisting of Poly(methyl methacrylate) (PMMA), Low Density Polyethylene (LDPE), Linear Low Density Polyethylene (LLDPE), High Density Polyethylene (HDPE), Polyethylene Terephthalate (PET), Polyethylene (PE), polycarbonate (PC), Polyethylene Terephthalate Glycol (PETG), Polystyrene (PS), Ethyl Vinyl Acetate (EVA), and polyethylene napthalate (PEN).

36. The method according to claim 26 further comprising the step of:

detecting said electromagnetic radiation in an optical feedback system wherein said perforation of said material layer is signaled to said optical feedback system thereby controlling said electromagnetic radiation means to substantially stop.

37. A disk for processing fluids comprising:

a first substrate comprising a plurality of first fluidic components;

a second substrate comprising a plurality of second fluidic components corresponding to the first fluidic components; and

a material layer separating the plurality of first fluidic components from the plurality of second fluidic components said material layer having an optical dye to absorb radiation at a selected portion wherein said absorption perforates said material layer at said selected portion allowing for fluid communication between said first fluidic components and said second fluidic components.