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Multi-dimensional electrophoresis apparatus

  • US 7,153,407 B2
  • Filed: 06/17/2002
  • Issued: 12/26/2006
  • Est. Priority Date: 11/08/1999
  • Status: Expired due to Term
First Claim
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1. An electrophoresis apparatus, comprising:

  • a transport passage having a sample inlet end and an outlet end;

    a first separation passage in fluid communication with and intersecting the transport passage at a first intersection and crossing over the transport passage;

    the first separation passage having a buffer solution inlet end;

    first affinity means for attracting and concentrating in the first intersection a first analyte of interest from a sample introduced into the transport passage and allowing the concentrated first analyte of interest to be subsequently conveyed by at least electrophoresis migration via the first separation passage to a detector system which identifies and characterizes analytes of interest;

    a second separation passage in fluid communication with and intersecting the transport passage at a second intersection which is downstream of the first intersection and crossing over the transport passage;

    the second separation passage having a buffer solution inlet end; and

    second affinity means for attracting and concentrating in the second intersection a second analyte of interest from the introduced sample and allowing the concentrated second analyte of interest to be subsequently conveyed by at least electrophoresis migration via the second separation passage to the detector system.

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