Flow-through optical assay devices providing laminar flow of fluid samples, and methods of construction thereof
First Claim
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1. An optical assay device for the detection of an analyte of interest in a fluid sample comprising:
- a support containing channels;
an optically functional layer containing channels, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, positioned on said support;
an attachment layer containing channels positioned on said optically functional layer; and
an analyte specific receptive layer containing channels positioned on said attachment layer,wherein said channels in said support, said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from the analyte specific receptive layer to the support.
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Abstract
An optical assay device for the detection of an analyte of interest in a sample comprising a support containing channels, an optically functional layer positioned on the support such that the optically functional layer and the support allow for laminar flow of the sample through layers of the device, an attachment layer positioned on the optically functional layer, and an analyte specific receptive layer positioned on the attachment layer.
55 Citations
40 Claims
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1. An optical assay device for the detection of an analyte of interest in a fluid sample comprising:
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a support containing channels; an optically functional layer containing channels, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, positioned on said support; an attachment layer containing channels positioned on said optically functional layer; and an analyte specific receptive layer containing channels positioned on said attachment layer, wherein said channels in said support, said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from the analyte specific receptive layer to the support. - View Dependent Claims (7, 8, 9, 10, 11, 12, 30, 31, 32, 35, 36, 39)
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2. An optical assay device for the detection of an analyte of interest in a fluid sample comprising:
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a support containing channels; an optically functional layer containing channels, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, positioned on said support; and an attachment layer containing channels positioned on said optically functional layer to provide nonspecific capture of said analyte, wherein said channels in said support, said optically functional layer and said attachment layer are positioned to allow fluid flow from the attachment layer to the support.
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3. An optical assay device for the detection of an analyte of interest in a fluid sample comprising:
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a porous support; an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, comprising discrete, optically functional particles embedded in said support configured and arranged to provide channels through said optically functional layer; an attachment layer containing channels positioned on said particles; and an analyte specific receptive layer containing channels positioned on said attachment layer, wherein said channels in said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from said analyte specific receptive layer to said attachment layer and to said optically functional layer wherein fluid enters pores of said porous support.
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4. An optical assay device for the detection of an analyte of interest in a sample comprising:
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a porous support; an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, comprising discrete, optically functional particles embedded in said support configured and arranged to provide channels through said optically functional layer; and an attachment layer containing channels positioned on said particles to provide nonspecific capture of said analyte, wherein said channels in said optically functional layer and said attachment layer are positioned to allow fluid flow from the attachment layer to said optically functional layer wherein fluid enters pores of said porous support.
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5. An optical assay device for the detection of an analyte of interest in a sample comprising:
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a porous support; an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, containing channels positioned on said support; an attachment layer containing channels positioned on said optically functional layer; and an analyte specific receptive layer containing channels positioned on said attachment layer, wherein said channels in said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from said analyte specific receptive layer to said attachment layer and to said optically functional layer wherein fluid enters pores of said porous support.
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6. An optical assay device for the detection of an analyte of interest in a sample comprising:
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a porous support; an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, containing channels positioned on said support; and an attachment layer containing channels positioned on said optically functional layer to provide nonspecific capture of said analyte, wherein said channels in said optically functional layer and said attachment layer are positioned to allow fluid flow from said attachment layer to said optically functional layer wherein fluid enters pores of said porous support.
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13. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a support comprising channels; providing an optically functional layer containing channels, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, on said support; providing an attachment layer containing channels on said optically functional layer, and providing an analyte specific receptive layer containing channels on said attachment layer, wherein said channels in said support, said optically functional layer, said attachment layer and said an analyte specific receptive layer are positioned to allow fluid flow from the analyte specific receptive layer to the support.
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14. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a support comprising channels; providing an optically functional layer containing channels, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, on said support; and providing an attachment layer containing channels on said optically functional layer to provide nonspecific capture of said analyte, wherein said channels in said support, said optically functional layer and said attachment layer are positioned to allow fluid flow from the attachment layer to the support. - View Dependent Claims (33, 34)
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15. A composition comprising:
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a support comprising channels or a porous support, and an optically functional layer containing channels positioned on said support, said optically functional layer having an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding; wherein said channels in said optically functional layer are positioned to allow fluid flow to the support either through channels in the support or via pores in the porous support. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 37, 38, 40)
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26. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a porous support; providing an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, comprising discrete, optically functional particles embedded in said support configured and arranged to provide channels through said optically functional layer; providing an attachment layer containing channels positioned on said particles; and providing an analyte specific receptive layer containing channels positioned on said attachment layer, wherein said channels in said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from said analyte specific receptive layer to said attachment layer and to said optically functional layer wherein fluid enters pores of said porous support.
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27. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a porous support; providing an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, comprising discrete, optically functional particles embedded in said support configured and arranged to provide channels through said optically functional layer; and providing an attachment layer containing channels positioned on said particles to provide nonspecific capture of said analyte, wherein said channels in said optically functional layer and said attachment layer are positioned to allow fluid flow from the attachment layer to said optically functional layer wherein fluid enters pores of said porous support.
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28. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a porous support, providing an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, containing channels positioned on said support; providing an attachment layer containing channels positioned on said optically functional layer; and providing an analyte specific receptive layer containing channels positioned on said attachment layer, wherein said channels in said optically functional layer, said attachment layer and said analyte specific receptive layer are positioned to allow fluid flow from said analyte specific receptive layer to said attachment layer and to said optically functional layer wherein fluid enters pores of said porous support.
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29. Method for constructing an optical assay device with laminar flow properties, comprising the steps of:
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providing a porous support; providing an optically functional layer, an optical property of which is detectably altered upon a change in mass on said optically functional layer related to analyte binding, containing channels positioned on said support; and providing an attachment layer containing channels positioned on said optically functional layer to provide nonspecific capture of said analyte, wherein said channels in said optically functional layer and said attachment layer are positioned to allow fluid flow from said attachment layer to said optically functional layer wherein fluid enters pores of said porous support.
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Specification