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Methods and compositions for determination of glycated proteins

  • US 7,153,666 B2
  • Filed: 07/17/2003
  • Issued: 12/26/2006
  • Est. Priority Date: 07/17/2003
  • Status: Active Grant
First Claim
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1. An isolated chimeric protein having enzymatic activity of an amadoriase, which chimeric protein comprises, from N-terminus to C-terminus:

  • a) a first peptidyl fragment comprising a bacterial leader sequence comprising the amino acid sequence set forth in SEQ ID NO;

    1;

    b) a second peptidyl fragment comprising an amadoriase comprising the amino acid sequence set forth in SEQ ID NO;

    3; and

    c) a third peptidyl fragment comprising a second bacterial leader sequence comprising the amino acid sequence set forth in SEQ ID NO;

    4.

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