Tamoxifen and 4-hydroxytamoxifen-activated system for regulated production of proteins in eukaryotic cells
First Claim
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1. An isolated polynucleotide, comprising:
- a first polynucleotide; and
a second polynucleotide operably linked to the first polynucleotide;
wherein when the first polynucleotide is inserted into a promoter region of a gene, enhances transcription of the gene, when the first polynucleotide is bound by a mutant estrogen receptor to which is bound 4-hydroxytamoxifen,the first polynucleotide comprises nucleotides 2–
6 and 10–
14 of SEQ ID NO;
75, andthe mutant estrogen receptor has the amino acid sequence of SEQ ID NO;
4.
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Abstract
Novel tamoxifen inducible and ICI 182,780 repressible expression systems comprising mutant estrogen receptors and mutant estrogen response element are disclosed. Such systems have a wide variety of applications, including gene therapy and in vivo and in vitro expression, as well as their use in transgenic animals.
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Citations
30 Claims
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1. An isolated polynucleotide, comprising:
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a first polynucleotide; and a second polynucleotide operably linked to the first polynucleotide; wherein when the first polynucleotide is inserted into a promoter region of a gene, enhances transcription of the gene, when the first polynucleotide is bound by a mutant estrogen receptor to which is bound 4-hydroxytamoxifen, the first polynucleotide comprises nucleotides 2–
6 and 10–
14 of SEQ ID NO;
75, andthe mutant estrogen receptor has the amino acid sequence of SEQ ID NO;
4. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification