Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor
First Claim
Patent Images
1. A method of detecting a change in activity of one or more enzymes or binding partners due to one or more molecules, wherein the enzymes or binding partners cleave or bind one or more specific binding substances, wherein the one or more specific binding substances are immobilized on a surface of a colorimetric resonant reflectance optical biosensor at one or more distinct locations, comprising:
- a. detecting a colorimetric resonant reflectance optical PWV (peak wavelength value) of the one or more distinct locations;
b. applying the one or more molecules to the one or more distinct location;
c. applying the one or more enzymes or binding partners to the one or more distinct locations;
d. detecting the colorimetric resonant reflectance optical PWV of the one or more distinct locations;
e. comparing the PWV from step (a) with the PWV from step (d); and
wherein a change in activity of one or more enzymes or binding partners due to one or more molecules is detected.
3 Assignments
0 Petitions
Accused Products
Abstract
Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.
184 Citations
10 Claims
-
1. A method of detecting a change in activity of one or more enzymes or binding partners due to one or more molecules, wherein the enzymes or binding partners cleave or bind one or more specific binding substances, wherein the one or more specific binding substances are immobilized on a surface of a colorimetric resonant reflectance optical biosensor at one or more distinct locations, comprising:
-
a. detecting a colorimetric resonant reflectance optical PWV (peak wavelength value) of the one or more distinct locations; b. applying the one or more molecules to the one or more distinct location; c. applying the one or more enzymes or binding partners to the one or more distinct locations; d. detecting the colorimetric resonant reflectance optical PWV of the one or more distinct locations; e. comparing the PWV from step (a) with the PWV from step (d); and wherein a change in activity of one or more enzymes or binding partners due to one or more molecules is detected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
Specification